ArticleSensors (Basel, Switzerland)2008
An Electrochemical Detection of Metallothioneins at the Zeptomole Level in Nanolitre Volumes.
Article in Sensors (Basel, Switzerland), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Metallothionein modulation in relation to cadmium bioaccumulation and age-dependent sensitivity of Chironomus riparius larvae.Environmental science and pollution research international · 2016Article
- Improved Electrochemical Detection of Zinc Ions Using Electrode Modified with Electrochemically Reduced Graphene Oxide.Materials (Basel, Switzerland) · 2016Article
- Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus.International journal of environmental research and public health · 2014Article
- Serum metallothioneins in childhood tumours-a potential prognostic marker.International journal of molecular sciences · 2013Article
- Bio-sensing of cadmium(II) ions using Staphylococcus aureus.Sensors (Basel, Switzerland) · 2011Article
- Zeptomole electrochemical detection of metallothioneins.PloS one · 2010Article
- An adsorptive transfer technique coupled with brdicka reaction to reveal the importance of metallothionein in chemotherapy with platinum based cytostatics.International journal of molecular sciences · 2010Article
- Changes in metallothionein level in rat hepatic tissue after administration of natural mouldy wheat.International journal of molecular sciences · 2009Article
- Effect of cadmium chloride on metallothionein levels in carp.Sensors (Basel, Switzerland) · 2009Article
- Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdickás reaction and Quartz Crystal Microbalance.Sensors (Basel, Switzerland) · 2009Article
- Determination of Vitamin C (Ascorbic Acid) Using High Performance Liquid Chromatography Coupled with Electrochemical Detection.Sensors (Basel, Switzerland) · 2008Article
- Utilization of Electrochemical Sensors and Biosensors in Biochemistry and Molecular Biology.Sensors (Basel, Switzerland) · 2008Article
- A Determination of Metallothionein in Larvae of Freshwater Midges (Chironomus riparius) Using Brdicka Reaction.Sensors (Basel, Switzerland) · 2008Article
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5 authors.
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Abstract
An Electrochemical Detection of Metallothioneins at the Zeptomole Level in Nanolitre VolumesWe report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer. Based on the results obtained it can be concluded that the improved technique can be used to detect a thiolprotein in very low sample volumes and can also prevent interferences during the washing and transferring step.
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