ArticleJournal of tissue engineering and regenerative medicine2017
Silk fibroin scaffolds with muscle-like elasticity support in vitro differentiation of human skeletal muscle cells.
Article in Journal of tissue engineering and regenerative medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Mechanical and structural responses of silk fibroin scaffolds to MechanoCulture T6® bioreactor loading and enzymatic degradation.Journal of the mechanical behavior of biomedical materials · 2026Article
- Re-innervation of neuromuscular junctions by a conductive polypyrrole/silk fibroin/GelMA hydrogel facilitated functional skeletal muscle regeneration following volumetric muscle loss.Journal of orthopaedic translation · 2026Article
- Collagen Scaffold Viscoelasticity Regulates Muscle Cell Phenotype.Advanced healthcare materials · 2026Article
- Edible Scaffolds for Cultivated Meat Production.Advances in biochemical engineering/biotechnology · 2026Review
- Biomimetic Scaffolds and Extracellular Matrix-Based Strategies for Myofiber Regeneration in Volumetric Muscle Loss.Drug design, development and therapy · 2026Review
- Keratinocyte self-renewal and differentiation is dictated by extrinsic signals from dermal extracellular matrices.Open biology · 2025Article
- Development of silk microfiber-reinforced bioink for muscle tissue engineering and in situ printing by a handheld 3D printer.Biomaterials advances · 2025Article
- Aligned Collagen Sponges with Tunable Pore Size for Skeletal Muscle Tissue Regeneration.Journal of functional biomaterials · 2023Article
- The Use of Collagen Methacrylate in Actuating Polyethylene Glycol Diacrylate-Acrylic Acid Scaffolds for Muscle Regeneration.Annals of biomedical engineering · 2023Article
- Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications.Biomimetics (Basel, Switzerland) · 2023Review
- Porous biomaterial scaffolds for skeletal muscle tissue engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Multiple Cell Cultures for MRI Analysis.International journal of molecular sciences · 2022Review
- Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration.Polymers · 2022Article
- Article
- Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Mobilizing Endogenous Repair Through Understanding Immune Reaction With Biomaterials.Frontiers in bioengineering and biotechnology · 2021Review
- Next Stage Approach to Tissue Engineering Skeletal Muscle.Bioengineering (Basel, Switzerland) · 2020Review
- Design of Bio-Conjugated Hydrogels for Regenerative Medicine Applications: From Polymer Scaffold to Biomolecule Choice.Molecules (Basel, Switzerland) · 2020Review
- Vascularized and Innervated Skeletal Muscle Tissue Engineering.Advanced healthcare materials · 2020Review
- Growth factor-free salt-leached silk scaffolds for differentiating endothelial cells.Journal of materials chemistry. B · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human adult skeletal muscle has a limited ability to regenerate after injury and therapeutic options for volumetric muscle loss are few. Technologies to enhance regeneration of tissues generally rely upon bioscaffolds to mimic aspects of the tissue extracellular matrix (ECM). In the present study, silk fibroins from four Lepidoptera (silkworm) species engineered into three-dimensional scaffolds were examined for their ability to support the differentiation of primary human skeletal muscle myoblasts. Human skeletal muscle myoblasts (HSMMs) adhered, spread and deposited extensive ECM on all the scaffolds, but immunofluorescence and quantitative polymerase chain reaction analysis of gene expression revealed that myotube formation occurred differently on the various scaffolds. Bombyx mori fibroin scaffolds supported formation of long, well-aligned myotubes, whereas on Antheraea mylitta fibroin scaffolds the myotubes were thicker and shorter. Myotubes were oriented in two perpendicular layers on Antheraea assamensis scaffolds, and scaffolds of Philosamia/Samia ricini (S. ricini) fibroin poorly supported myotube formation. These differences were not caused by fibroin composition per se, as HSMMs adhered to, proliferated on and formed striated myotubes on all four fibroins presented as two-dimensional fibroin films. The Young's modulus of A. mylitta and B. mori scaffolds mimicked that of normal skeletal muscle, but A. assamensis and S. ricini scaffolds were more flexible. The present study demonstrates that although myoblasts deposit matrix onto fibroin scaffolds and create a permissive environment for cell proliferation, a scaffold elasticity resembling that of normal muscle is required for optimal myotube length, alignment, and maturation. © 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. StartCopTextStartCopText© 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.