ArticleCurrent genetics2017
The CreB deubiquitinating enzyme does not directly target the CreA repressor protein in Aspergillus nidulans.
Article in Current genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Regulation of genes encoding polysaccharide-degrading enzymes in Penicillium.Applied microbiology and biotechnology · 2024Review
- A novel luciferase-based reporter tool to monitor the dynamics of carbon catabolite repression in filamentous fungi.Microbial biotechnology · 2024Article
- Regulation of nutrient utilization in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- Genome-wide transcription landscape of citric acid producingFrontiers in bioengineering and biotechnology · 2023Article
- cAMP signaling factors regulate carbon catabolite repression of hemicellulase genes in Aspergillus nidulans.AMB Express · 2022Article
- Post-translational modifications drive secondary metabolite biosynthesis in Aspergillus: a review.Environmental microbiology · 2022Review
- Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle ofJournal of fungi (Basel, Switzerland) · 2021Article
- Aflatoxin Biosynthesis, Genetic Regulation, Toxicity, and Control Strategies: A Review.Journal of fungi (Basel, Switzerland) · 2021Review
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- Induction and Repression of Hydrolase Genes inFrontiers in microbiology · 2021Review
- Genome-wide identification of genes encoding putative secreted E3 ubiquitin ligases and functional characterization of PbRING1 in the biotrophic protist Plasmodiophora brassicae.Current genetics · 2019Article
- CreA-independent carbon catabolite repression of cellulase genes by trimeric G-protein and protein kinase A in Aspergillus nidulans.Current genetics · 2019Article
- Article
- Protein O-mannosyltransferases are required for sterigmatocystin production and developmental processes in Aspergillus nidulans.Current genetics · 2018Article
- Regulation ofmBio · 2018Article
- Overview of carbon and nitrogen catabolite metabolism in the virulence of human pathogenic fungi.Molecular microbiology · 2018Review
- Carbon Catabolite Repression in Filamentous Fungi.International journal of molecular sciences · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ubiquitination/deubiquitination pathways are now recognized as key components of gene regulatory mechanisms in eukaryotes. The major transcriptional repressor for carbon catabolite repression in Aspergillus nidulans is CreA, and mutational analysis led to the suggestion that a regulatory ubiquitination/deubiquitination pathway is involved. A key unanswered question is if and how this pathway, comprising CreB (deubiquitinating enzyme) and HulA (ubiquitin ligase) and other proteins, is involved in the regulatory mechanism. Previously, missense alleles of creA and creB were analysed for genetic interactions, and here we extended this to complete loss-of-function alleles of creA and creB, and compared morphological and biochemical phenotypes, which confirmed genetic interaction between the genes. We investigated whether CreA, or a protein in a complex with it, is a direct target of the CreB deubiquitination enzyme, using co-purifications of CreA and CreB, first using strains that overexpress the proteins and then using strains that express the proteins from their native promoters. The Phos-tag system was used to show that CreA is a phosphorylated protein, but no ubiquitination was detected using anti-ubiquitin antibodies and Western analysis. These findings were confirmed using mass spectrometry, which confirmed that CreA was differentially phosphorylated but not ubiquitinated. Thus, CreA is not a direct target of CreB, and nor are proteins that form part of a stable complex with CreA a target of CreB. These results open up new questions regarding the molecular mechanism of CreA repressing activity, and how the ubiquitination pathway involving CreB interacts with this regulatory network.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.