Evidence map›Paper›PMID 27878499›Full record

ArticleMolecular and cellular biochemistry2017

Therapeutic potential of ADAM17 modulation in gastric cancer through regulation of the EGFR and TNF-α signalling pathways.

Jinbing Sun, Jianlong Jiang, Kuangyi Lu, Qiao Chen, Danhao Tao, Zhong Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular and cellular biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Jinbing SunMedical College of Soochow University, Suzhou, 215006, China.
Jianlong JiangDepartment of General Surgery, Changshu No. 1 People's Hospital Affiliated to Soochow University, Changshu, 215500, Jiangsu, China.
Kuangyi LuDepartment of General Surgery, Changshu No. 1 People's Hospital Affiliated to Soochow University, Changshu, 215500, Jiangsu, China.
Qiao ChenDepartment of General Surgery, Changshu No. 1 People's Hospital Affiliated to Soochow University, Changshu, 215500, Jiangsu, China.
Danhao TaoDepartment of General Surgery, Changshu No. 1 People's Hospital Affiliated to Soochow University, Changshu, 215500, Jiangsu, China.
Zhong ChenMedical College of Soochow University, Suzhou, 215006, China. chenz9806@163.com.
Changshu No.1 People's Hospital · CNSoochow University · CNNantong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A disintegrin and metalloproteinase 17 (ADAM17) is highly expressed in various tumours and affects tumour progression. In this study, ADAM17 expression in 60 gastric cancer and 20 normal gastric mucosal tissues was assessed using immunohistochemistry. ADAM17 expression was higher in gastric cancer tissues than in normal gastric mucosal tissues (P < 0.0005). A significant relationship was identified between ADAM17 expression and the depth of tumour invasion, metastasis, and carcinoma stage. Furthermore, the effects of ADAM17 knockdown on the proliferation, cell invasion, and apoptosis of human gastric carcinoma cells (SGC-7901) were determined. SGC-7901 cells were transfected with ADAM17-shRNA, and cell proliferation and migration were assessed using CCK-8 and transwell assays, respectively, to evaluate the role of ADAM17 in tumour proliferation and invasion. Furthermore, the EGFR signalling pathway, the cell membrane receptor-bound TNF-α level, and apoptosis were evaluated by western blotting and flow cytometry. The inhibition of cell proliferation and invasion was observed in the ADAM17 knockdown cells, which was associated with modulation of the EGFR signalling pathway. Apoptosis was increased, and TNF-α signalling was attenuated in the ADAM17 knockdown cells. Our study demonstrated that ADAM17 over-expression in gastric cancer tissues was closely associated with tumour proliferation, invasion, and apoptosis.

Indexed as

Gene Expression Regulation, EnzymologicGene Expression Regulation, NeoplasticSignal TransductionADAM17 ProteinCell Line, TumorCell ProliferationErbB ReceptorsGene Knockdown TechniquesHumansMaleNeoplasm InvasivenessNeoplasm MetastasisNeoplasm ProteinsRNA, Small InterferingStomach NeoplasmsTumor Necrosis Factor-alphaADAM17 ProteinADAM17 protein, humanEGFR protein, humanErbB ReceptorsNeoplasm ProteinsRNA, Small InterferingTumor Necrosis Factor-alphaADAM17EGFRGastric cancerTNF-α

Identifiers

PMID27878499
OpenAlexW2550143520

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.