ArticleJournal of applied genetics2017
Evaluation of stability and validation of reference genes for RT-qPCR expression studies in rice plants under water deficit.
Article in Journal of applied genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A banana susceptibility gene underlying fusarium wilt: validation as a target for disease resistance.Molecular biology reports · 2026Article
- Identification of Reference Genes for RT-qPCR Assays inCurrent issues in molecular biology · 2026Article
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
- Ectopic expression of maize gene ZmDUF1645 in rice affects rice grain traits and cold tolerance.BMC plant biology · 2025Article
- Selection of stable reference genes for accurate reverse-transcription quantitative PCR in cotton-herbivore studies using virus-induced gene silencing.Scientific reports · 2025Article
- Selection of stable reference genes in prunus persica fruit infected with monilinia laxa for normalisation of RT-qPCR gene expression data.Scientific reports · 2025Article
- Development and evaluation of suitable reference genes for qRT-PCR normalization of hybrids derived from Lycium barbarum and Lycium ruthenicum.Molecular biology reports · 2024Article
- Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Integrated transcriptome and metabolome analysis of salinity tolerance in response to foliar application of choline chloride in rice (Frontiers in plant science · 2024Article
- Identification of Reference Genes for Precise Expression Analysis during Germination inInternational journal of molecular sciences · 2023Article
- Selection of reference genes for RT-qPCR analysis of rice with Rhizoctonia solani infection and biocontrol PGPR/KSi application.Molecular biology reports · 2023Article
- Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Identification of Reliable Reference Genes under Different Stresses and in Different Tissues ofGenes · 2022Article
- Validation of Reference Genes for Studying Different Abiotic Stresses in Oat (Plants (Basel, Switzerland) · 2021Article
- Identification and selection of reference genes for gene expression analysis by quantitative real-time PCR in Suaeda glauca's response to salinity.Scientific reports · 2021Article
- Article
- Identification and validation of superior housekeeping gene(s) for qRT-PCR data normalization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Evaluation of reference genes and expression of key genes involved in the isoprenoid metabolic pathway of rice leaves after infection by the Southern rice black-streaked dwarf virus.Molecular biology reports · 2019Article
- Analysis of differential gene expression and alternative splicing is significantly influenced by choice of reference genome.RNA (New York, N.Y.) · 2019Article
- Selection of the optimal reference genes for expression analyses in different materials ofPlant methods · 2019Article
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7 authors.
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Abstract
Many studies use strategies that allow for the identification of a large number of genes expressed in response to different stress conditions to which the plant is subjected throughout its cycle. In order to obtain accurate and reliable results in gene expression studies, it is necessary to use reference genes, which must have uniform expression in the majority of cells in the organism studied. RNA isolation of leaves and expression analysis in real-time quantitative polymerase chain reaction (RT-qPCR) were carried out. In this study, nine candidate reference genes were tested, actin 11 (ACT11), ubiquitin conjugated to E2 enzyme (UBC-E2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta tubulin (β-tubulin), eukaryotic initiation factor 4α (eIF-4α), ubiquitin 10 (UBQ10), ubiquitin 5 (UBQ5), aquaporin TIP41 (TIP41-Like) and cyclophilin, in two genotypes of rice, AN Cambará and BRS Querência, with different levels of soil moisture (20%, 10% and recovery) in the vegetative (V5) and reproductive stages (period preceding flowering). Currently, there are different softwares that perform stability analyses and define the most suitable reference genes for a particular study. In this study, we used five different methods: geNorm, BestKeeper, ΔCt method, NormFinder and RefFinder. The results indicate that UBC-E2 and UBQ5 can be used as reference genes in all samples and softwares evaluated. The genes β-tubulin and eIF-4α, traditionally used as reference genes, along with GAPDH, presented lower stability values. The gene expression of basic leucine zipper (bZIP23 and bZIP72) was used to validate the selected reference genes, demonstrating that the use of an inappropriate reference can induce erroneous results.
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