Evidence map›Paper›PMID 27878106›Full record

ArticleFrontiers in oncology2016

Multiparameter Evaluation of the Heterogeneity of Circulating Tumor Cells Using Integrated RNA

Yongqi Wu, Kyoung-Joo Jenny Park, Clayton Deighan, Peter Amaya, Brandon Miller, Quintin Pan, Maciej Zborowski, Maryam Lustberg, Jeffery Chalmers

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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  8. Liquid biopsy: current technology and clinical applications.Journal of hematology & oncology · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yongqi WuWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University , Columbus, OH , USA.
Kyoung-Joo Jenny ParkWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University , Columbus, OH , USA.
Clayton DeighanWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University , Columbus, OH , USA.
Peter AmayaDepartment of Biomedical Engineering, The Ohio State University , Columbus, OH , USA.
Brandon MillerWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University , Columbus, OH , USA.
Quintin PanDepartment of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital and Solove Research Institute, Wexner Medical Center at The Ohio State University , Columbus, OH , USA.
Maciej ZborowskiDepartment of Biomedical Engineering, Cleveland Clinic , Cleveland, OH , USA.
Maryam LustbergBreast Medical Oncology, Department of Internal Medicine, James Cancer Hospital, Ohio State University Comprehensive Cancer Center , Columbus, OH , USA.
Jeffery ChalmersWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA; Analytical Cytometry Shared Resource, Ohio State University Comprehensive Cancer Center, Columbus, OH, USA.

Funding

Magnetophoretic Cell Sorting and AnalysisR01CA062349 · NCI · CLEVELAND CLINIC LERNER COM-CWRU · PI ZBOROWSKI, MACIEJ · 1994 to 2015
$5.5M
NCI NIH HHS R01 CA062349
6 · The paper itself

Abstract

Circulating tumor cells (CTCs) are routinely identified as cytokeratin (CK)-positive, epithelial cell adhesion molecule (EpCAM)-positive, and CD45-negative and are enriched based on EpCAM. However, there are a number of methodological challenges regarding both isolation and characterization of these rare CTCs including downregulation or absence of EpCAM in a variety of solid tumors leading to the omission of subpopulations of CTCs, difficulties in analyzing RNA and protein targets in CTCs due to the rarity of these cells, and low levels of targets and technological limitations of visualizing the targets of interest on each individual cell. Building on our previous CTC research on CD45-based negative magnetic separation and four-color fluorescent immunocytochemical (ICC) staining, RNA

Indexed as

circulating tumor cellsfluorescent labelingintegrated RNA ISH and ICC stainingmultiparameter analysisnegative magnetic enrichment

Identifiers

PMID27878106
PMCPMC5099140

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.