Evidence map›Paper›PMID 27877910›Full record

ArticleScience and technology of advanced materials2016

Identifying DNA methylation in a nanochannel.

Xiaoyin Sun, Takao Yasui, Takeshi Yanagida, Noritada Kaji, Sakon Rahong, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science and technology of advanced materials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Xiaoyin SunDepartment of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan; ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya, Japan.
Takao YasuiDepartment of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan; ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya, Japan; Japan Science and Technology Agency (JST), PRESTO, Saitama, Japan.
Takeshi YanagidaInstitute of Materials Chemistry and Engineering, Kyushu University, Fukuoka, Japan; Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
Noritada KajiDepartment of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan; ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya, Japan.
Sakon RahongDepartment of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan; ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya, Japan.
Masaki KanaiInstitute of Materials Chemistry and Engineering, Kyushu University , Fukuoka , Japan.
Kazuki NagashimaInstitute of Materials Chemistry and Engineering, Kyushu University , Fukuoka , Japan.
Tomoji KawaiInstitute of Scientific and Industrial Research, Osaka University , Osaka , Japan.
Yoshinobu BabaDepartment of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan; ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya, Japan; Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan.
Nagoya University · JPKyushu University · JPOsaka Research Institute of Industrial Science and Technology · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA methylation is a stable epigenetic modification, which is well known to be involved in gene expression regulation. In general, however, analyzing DNA methylation requires rather time consuming processes (24-96 h) via DNA replication and protein modification. Here we demonstrate a methodology to analyze DNA methylation at a single DNA molecule level without any protein modifications by measuring the contracted length and relaxation time of DNA within a nanochannel. Our methodology is based on the fact that methylation makes DNA molecules stiffer, resulting in a longer contracted length and a longer relaxation time (a slower contraction rate). The present methodology offers a promising way to identify DNA methylation without any protein modification at a single DNA molecule level within 2 h.

Indexed as

102 Porous / Nanoporous / Nanostructured materials30 Bio-inspired and biomedical materialsDNA contractionDNA methylationnanochannelsingle DNA molecule

Identifiers

PMID27877910
PMCPMC5102024
OpenAlexW2509215892

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.