ArticleScience and technology of advanced materials2012
Quantitative comparison between poly(L-arginine) and poly(L-lysine) at each step of polyplex-based gene transfection using a microinjection technique.
Article in Science and technology of advanced materials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 21 citations in OpenAlex.
- Review
- Dynamic Covalent Boronate Chemistry Accelerates the Screening of Polymeric Gene Delivery Vectors viaJournal of the American Chemical Society · 2024Article
- Efficient Induction of Antigen-Specific CD8Pharmaceutics · 2022Article
- Poly-L-Arginine Molecule Properties in Simple Electrolytes: Molecular Dynamic Modeling and Experiments.International journal of environmental research and public health · 2022Article
- Interactions of Casein and Polypeptides in Multilayer Films Studied by FTIR and Molecular Dynamics.Polymers · 2019Article
- Binary Targeting of siRNA to Hematologic Cancer CellsAdvanced functional materials · 2019Article
- Comparative study of guanidine-based and lysine-based brush copolymers for plasmid delivery.Biomaterials science · 2013Article
- Facile functionalization of polyesters through thiol-yne chemistry for the design of degradable, cell-penetrating and gene delivery dual-functional agents.Biomacromolecules · 2012Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among the well-studied polypeptide-type gene carriers, transfection efficiency is empirically known to be higher for poly(L-arginine) (PR) than poly(L-lysine) (PK). The big difference between PR and PK should be determined at one of the intracellular trafficking steps based on the different charge densities, structures or PKa values. However, the endosomal escape and the intranuclear transcription efficiency in living cells have not been clarified yet. In this study, a novel method for quantifying the intranuclear transcription efficiency and the nuclear transport of the polyplex is established based on the nuclear and the cytosolic microinjection technique, and the results for PK and PR with different molecular weights (MWs) are compared in living cells. The intranuclear transcription efficiency is the same in PR and PK and it decreases rapidly with increasing MW, in spite of the commonly measured transfection efficiency. The transcription efficiency is strongly suppressed at high MW and strongly correlates with the polyplex forming ability expressed as a critical ratio of the number of polypeptide cationic groups to the number of pDNA anionic groups. When considered with the results of the cellular uptake and
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