ArticleFrontiers in plant science2016
Selection of Reference Genes for RT-qPCR Analysis in
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Selection and Validation of Stable Reference Genes for RT-qPCR inInternational journal of molecular sciences · 2026Article
- Article
- Functions of Insulin-like Peptide Genes (Insects · 2024Article
- Article
- Comprehensive Screening and Validation of Stable Internal Reference Genes for Accurate qRT-PCR Analysis inInsects · 2024Article
- Article
- Evaluation of reference genes for quantitative expression analysis inFrontiers in physiology · 2024Article
- Selection and validation of optimal reference genes for RT-qPCR analyses inFrontiers in physiology · 2023Article
- Identification and Validation of Reference Genes for Expression Analysis Using qRT-PCR inInsects · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Identification and Validation of Reference Genes for Gene Expression Analysis inFrontiers in physiology · 2022Article
- Evaluation of Reference Genes inGenes · 2021Article
- Expression Profiles of Digestive Genes in the Gut and Salivary Glands of Tarnished Plant Bug (Hemiptera: Miridae).Journal of insect science (Online) · 2021Article
- Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird,Frontiers in physiology · 2021Article
- Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis inFrontiers in physiology · 2021Article
- Article
- Selection and Validation of Reference Genes for RT-qPCR Normalization inFrontiers in physiology · 2021Article
- Article
- Selection and validation of experimental condition-specific reference genes for qRT-PCR in Metopolophium dirhodum (Walker) (Hemiptera: Aphididae).Scientific reports · 2020Article
- Comprehensive Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR inGenes · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of genetically engineered plants that employ RNA interference (RNAi) to suppress invertebrate pests opens up new avenues for insect control. While this biotechnology shows tremendous promise, the potential for both non-target and off-target impacts, which likely manifest via altered mRNA expression in the exposed organisms, remains a major concern. One powerful tool for the analysis of these un-intended effects is reverse transcriptase-quantitative polymerase chain reaction, a technique for quantifying gene expression using a suite of reference genes for normalization. The seven-spotted ladybeetle
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.