ArticleScientific reports2016
Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Enzymatic quorum quenching alters the phyllosphere microbiome and suppresses a bacterial-induced plant disease.Scientific reports · 2026Article
- Impeding Quorum Sensing Among the Intestinal Microbiota Impacts the Metastatic Rate of Colorectal Cancer.Cancer medicine · 2025Article
- Quorum quenching enzymes disrupt bacterial communication in a sex- and dose-dependent manner.Animal models and experimental medicine · 2025Article
- Changes in Active Site Loop Conformation Relate to the Transition toward a Novel Enzymatic Activity.JACS Au · 2024Article
- Engineering quorum quenching acylases with improved kinetic and biochemical properties.Protein science : a publication of the Protein Society · 2024Article
- Mining thermophiles for biotechnologically relevant enzymes: evaluating the potential of European and Caucasian hot springs.Extremophiles : life under extreme conditions · 2023Review
- Tuning the Envelope Structure of Enzyme Nanoreactors for In Vivo Detoxification of Organophosphates.International journal of molecular sciences · 2023Article
- Field testing of an enzymatic quorum quencher coating additive to reduce biocorrosion of steel.Microbiology spectrum · 2023Article
- Emerging evidence-based innovative approaches to control catheter-associated urinary tract infection: a review.Frontiers in cellular and infection microbiology · 2023Review
- Lactonase-mediated inhibition of quorum sensing largely alters phenotypes, proteome, and antimicrobial activities inFrontiers in cellular and infection microbiology · 2023Article
- Evidence for Complex Interplay between Quorum Sensing and Antibiotic Resistance in Pseudomonas aeruginosa.Microbiology spectrum · 2022Article
- Applying molecular and phenotypic screening assays to identify efficient quorum quenching lactonases.Enzyme and microbial technology · 2022Article
- Quorum quenching enzymes and their effects on virulence, biofilm, and microbiomes: a review of recent advances.Expert review of anti-infective therapy · 2020Review
- Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control.The Journal of biological chemistry · 2020Review
- Engineering quorum quenching enzymes: progress and perspectives.Biochemical Society transactions · 2019Review
- Evaluation of biological and enzymatic quorum quencher coating additives to reduce biocorrosion of steel.PloS one · 2019Article
- Quorum Quenching Lactonase Strengthens Bacteriophage and Antibiotic Arsenal AgainstFrontiers in microbiology · 2019Article
- Signal Disruption Leads to Changes in Bacterial Community Population.Frontiers in microbiology · 2019Article
- Biotechnology of extremely thermophilic archaea.FEMS microbiology reviews · 2018Review
- Highly active enzymes by automated combinatorial backbone assembly and sequence design.Nature communications · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extremozymes have gained considerable interest as they could meet industrial requirements. Among these, SsoPox is a hyperthermostable enzyme isolated from the archaeon Sulfolobus solfataricus. This enzyme is a lactonase catalyzing the hydrolysis of acyl-homoserine lactones; these molecules are involved in Gram-negative bacterial communication referred to as quorum sensing. SsoPox exhibits promiscuous phosphotriesterase activity for the degradation of organophosphorous chemicals including insecticides and chemical warfare agents. Owing to its bi-functional catalytic abilities as well as its intrinsic stability, SsoPox is appealing for many applications, having potential uses in the agriculture, defense, food and health industries. Here we investigate the biotechnological properties of the mutant SsoPox-W263I, a variant with increased lactonase and phosphotriesterase activities. We tested enzyme resistance against diverse process-like and operating conditions such as heat resistance, contact with organic solvents, sterilization, storage and immobilization. Bacterial secreted materials from both Gram-negative and positive bacteria were harmless on SsoPox-W263I activity and could reactivate heat-inactivated enzyme. SsoPox showed resistance to harsh conditions demonstrating that it is an extremely attractive enzyme for many applications. Finally, the potential of SsoPox-W263I to be active at subzero temperature is highlighted and discussed in regards to the common idea that hyperthermophile enzymes are nearly inactive at low temperatures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.