Evidence map›Paper›PMID 27876681›Full record

ReviewMethods (San Diego, Calif.)2017

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development.

Min Shen, James Rusling, Chandra K Dixit

Abstract readReview
In one paragraph

Review in Methods (San Diego, Calif.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Journal of chemical information and modeling · 2025
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
  16. Enzymatic Protein Immobilization for Nanobody Array.Molecules (Basel, Switzerland) · 2024
    Article
  17. Article
  18. Article
  19. Review
  20. Review

8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Min Shen *Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060.
James RuslingDepartment of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060.
Chandra K Dixit *Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060.

Funding

Protein Biomarker Arrays for Personalized Treatment of Prostate CancerR01EB016707 · NIBIB · UNIVERSITY OF CONNECTICUT STORRS · PI RUSLING, JAMES F. · 2014 to 2017
$1.4M
Protein Biosensor Arrays Based on NanomaterialsR01EB014586 · NIBIB · UNIVERSITY OF CONNECTICUT STORRS · PI RUSLING, JAMES F. · 2011 to 2014
$1.4M
NIBIB NIH HHS R01 EB014586NIBIB NIH HHS R01 EB016707
6 · The paper itself

Abstract

Immobilized antibody systems are the key to develop efficient diagnostics and separations tools. In the last decade, developments in the field of biomolecular engineering and crosslinker chemistry have greatly influenced the development of this field. With all these new approaches at our disposal, several new immobilization methods have been created to address the main challenges associated with immobilized antibodies. Few of these challenges that we have discussed in this review are mainly associated to the site-specific immobilization, appropriate orientation, and activity retention. We have discussed the effect of antibody immobilization approaches on the parameters on the performance of an immunoassay.

Indexed as

Biosensing TechniquesImmunoassayAdsorptionAntibodies, ImmobilizedAntigensBinding SitesClick ChemistryCross-Linking ReagentsCycloaddition ReactionHumansImmunoconjugatesOligonucleotidesProtein BindingStaphylococcal Protein AAntibodies, ImmobilizedAntigensCross-Linking ReagentsImmunoconjugatesOligonucleotidesStaphylococcal Protein AAffinity tagsAntibodyAntibody-binding proteinAntibody fragmentsClick chemistryCycloadditionDiagnosticsImmobilizationMetal binding proteinNucleotide binding proteinOrientationPhysical adsorptionProtein ASeparationSite-selectiveSite-specificStaudinger ligationTherapeutics

Identifiers

PMID27876681
PMCPMC5374010

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.