ArticleMicrobial cell factories2016
Engineering an inducible gene expression system for Bacillus subtilis from a strong constitutive promoter and a theophylline-activated synthetic riboswitch.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed.
- Article
- Recombinant production of acidophilic L-arabinose isomerase from Lentilactobacillus parakefiri in Bacillus subtilis.Microbial cell factories · 2025Article
- Optimization Strategy of Expression Vectors and Regulatory Elements for Enhanced Protein Production inInternational journal of molecular sciences · 2025Review
- Engineering Bacillus subtilis for high-value bioproduction: recent advances and applications.Microbial cell factories · 2025Review
- A Strictly Inducible and Orthogonal Dre-rox System for Precise and Markerless Genome Editing inJournal of microbiology and biotechnology · 2025Article
- A cross-species inducible system for enhanced protein expression and multiplexed metabolic pathway fine-tuning in bacteria.Nucleic acids research · 2025Article
- An optimised promoter and signal peptide improves methionine production of a genetically engineeredFrontiers in microbiology · 2025Article
- Article
- Systematic Comparison and Rational Design of Theophylline Riboswitches for Effective Gene Repression.Microbiology spectrum · 2023Article
- The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids inFEMS microbes · 2023Article
- Influence of N-terminal His-tags on the production of recombinant proteins in the cytoplasm ofBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- An operator-based expression toolkit forProceedings of the National Academy of Sciences of the United States of America · 2022Article
- The multifunctionality of expression systems inExperimental biology and medicine (Maywood, N.J.) · 2021Review
- Attenuator LRR - a regulatory tool for modulating gene expression in Gram-positive bacteria.Microbial biotechnology · 2021Article
- The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis.Microbial cell factories · 2021Article
- An overview and future prospects of recombinant protein production in Bacillus subtilis.Applied microbiology and biotechnology · 2021Review
- Recent trends in biocatalysis.Chemical Society reviews · 2021Review
- The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis.Microbial cell factories · 2020Article
- Realization of Robust and Precise Regulation of Gene Expression by Multiple Sigma Recognizable Artificial Promoters.Frontiers in bioengineering and biotechnology · 2020Article
- Using the IPTG-Inducible Pgrac212 Promoter for Overexpression of Human Rhinovirus 3C Protease Fusions in the Cytoplasm of Bacillus subtilis Cells.Current microbiology · 2019Article
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7 authors.
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Abstract
backgroundSynthetic riboswitches have been increasingly used to control and tune gene expression in diverse organisms. Although a set of theophylline-responsive riboswitches have been developed for bacteria, fully functional expression elements mediated by synthetic riboswitches in Bacillus subtilis are rarely used because of the host-dependent compatibility between the promoters and riboswitches.
resultsA novel genetic element composed of the promoter P43 and a theophylline-riboswitch was developed and characterized in B. subtilis. When combined with a P43 promoter (P43'-riboE1), the theophylline-riboswitch successfully switched the constitutive expression pattern of P43 to an induced pattern. The expression mediated by the novel element could be activated at the translational level by theophylline with a relatively high induction ratio. The induction ratios for P43'-riboE1 by 4-mM theophylline were elevated during the induction period. The level of induced expression was dependent on the theophylline dose. Correspondingly, the induction ratios gradually increased in parallel with the elevated dose of theophylline. Importantly, the induced expression level was higher than three other strong constitutive promoters including P
conclusionThe constructed theophylline-inducible gene expression system has broad compatibility and robustness, which has great potential in over-production of pharmaceutical and industrial proteins and utilization in building more complex gene circuits.
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