ArticleScientific reports2016
Longitudinal metagenomic profiling of bovine milk to assess the impact of intramammary treatment using a third-generation cephalosporin.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed.
- Trial
- Variations in cow milk and teat skin microbiota across the lactation cycle with intramammary cephalosporin use at dry-off.Applied and environmental microbiology · 2026Article
- Age Influences the Bacterial Composition of Samples From Buffaloes in the Marajó Archipelago, Pará, Brazilian Amazon.Environmental microbiology reports · 2026Article
- Development of long-term antimicrobial resistance patterns of bovine mastitis pathogens in north-western Germany from 2005 to 2023.Veterinary research communications · 2026Article
- The Microbial Composition of Bovine Colostrum as Influenced by Antibiotic Treatment.Antibiotics (Basel, Switzerland) · 2025Article
- Antibiotic Treatment vs. Non-Antibiotic Treatment in Bovine Clinical Mastitis During Lactation with Mild and Moderate Severity.Antibiotics (Basel, Switzerland) · 2025Review
- Use of approved cefazolin based intramammary dry goat treatment and its effects on the milk microbiota.Scientific reports · 2025Article
- Resistome and microbiome profiling of bovine milk following antimicrobial dry cow therapy: insights from short- and long-read metagenomic sequencing.Frontiers in microbiomes · 2025Article
- Article
- The occurrence ofmSystems · 2024Article
- An in vitro evaluation of the effect of antimicrobial treatment on bovine mammary microbiota.Scientific reports · 2024Article
- Discriminating bovine mastitis pathogens by combining loop-mediated isothermal amplification and amplicon-binding split trehalase assay.Frontiers in veterinary science · 2024Article
- Persistent effects of intramammary ceftiofur treatment on the gut microbiome and antibiotic resistance in dairy cattle.Animal microbiome · 2023Article
- Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis.Animals : an open access journal from MDPI · 2023Article
- Microbiota and Resistome Analysis of Colostrum and Milk from Dairy Cows Treated with and without Dry Cow Therapies.Antibiotics (Basel, Switzerland) · 2023Article
- Effect of the Selective Dry Cow Therapy on Udder Health and Milk Microbiota.Antibiotics (Basel, Switzerland) · 2023Article
- Absence of changes in the milk microbiota during Escherichia coli endotoxin induced experimental bovine mastitis.Veterinary research · 2023Article
- Bovine Udder Health: From Standard Diagnostic Methods to New Approaches-A Practical Investigation of Various Udder Health Parameters in Combination with 16S rRNA Sequencing.Microorganisms · 2023Article
- Effects of Intramammary Antimicrobial Treatment on the Milk Microbiota Composition in Mild Clinical Bovine Mastitis Caused by Gram-Positive Bacteria.Animals : an open access journal from MDPI · 2023Article
- The bovine foot skin microbiota is associated with host genotype and the development of infectious digital dermatitis lesions.Microbiome · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antimicrobial usage in food animals has a direct impact on human health, and approximately 80% of the antibiotics prescribed in the dairy industry are used to treat bovine mastitis. Here we provide a longitudinal description of the changes in the microbiome of milk that are associated with mastitis and antimicrobial therapy. Next-generation sequencing, 16 S rRNA gene quantitative real-time PCR, and aerobic culturing were applied to assess the effect of disease and antibiotic therapy on the milk microbiome. Cows diagnosed with clinical mastitis associated with Gram-negative pathogens or negative aerobic culture were randomly allocated into 5 days of Ceftiofur intramammary treatment or remained as untreated controls. Serial milk samples were collected from the affected quarter and the ipsilateral healthy quarter of the same animal. Milk from the mastitic quarter had a higher bacterial load and reduced microbial diversity compared to healthy milk. Resolution of the disease was accompanied by increases in diversity indexes and a decrease in pathogen relative abundance. Escherichia coli-associated mastitic milk samples had a remarkably distinct bacterial profile, dominated by Enterobacteriaceae, when compared to healthy milk. However, no differences were observed in culture-negative mastitis samples when compared to healthy milk. Antimicrobial treatment had no significant effect on clinical cure, bacteriological cure, pathogen clearance rate or bacterial load.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.