ArticleScientific reports2016
Development of Commercial Thermo-sensitive Genic Male Sterile Rice Accelerates Hybrid Rice Breeding Using the CRISPR/Cas9-mediated TMS5 Editing System.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed.
- OsRNase ZPlant communications · 2026Article
- An efficient and accurate method for identifying the fertility of PTGMS lines in rice and its application.Scientific reports · 2026Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Unraveling Advances in Rice Male Sterility Systems: From Genetic Basis to Hybrid Breeding Innovation.Plants (Basel, Switzerland) · 2026Review
- Traceless Regulation of Genetic Circuitry.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- CRISPR-driven innovations in rice (Oryza sativa L.) breeding: precision development of male sterile lines.Molecular biology reports · 2025Review
- The OsRqc1-OsVms1 module regulates the temperature threshold in thermo-sensitive genic male-sterile rice lines.Science China. Life sciences · 2025Article
- Genome-wide identification of the Medicago sativa L. MYB family and its transcriptional dynamics during pollen development.BMC plant biology · 2025Article
- tRNA selectivity during ribosome-associated quality control regulates the critical sterility-inducing temperature in two-line hybrid rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Synthetic biology and artificial intelligence in crop improvement.Plant communications · 2025Review
- Article
- Unraveling the genetic and molecular bases of heterosis in a TGMS-based two-line rice hybrid derived FFrontiers in plant science · 2025Article
- Identification of U6 Promoter and Establishment of Gene-Editing System inPlants (Basel, Switzerland) · 2024Article
- CRISPR/Cas9 mediated genome editing for crop improvement against Abiotic stresses: current trends and prospects.Functional & integrative genomics · 2024Review
- Integrating physiological and multi-omics methods to elucidate heat stress tolerance for sustainable rice production.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Article
- Review
- Hybrid Rice Production: A Worldwide Review of Floral Traits and Breeding Technology, with Special Emphasis on China.Plants (Basel, Switzerland) · 2024Review
- Breeding rice for yield improvement through CRISPR/Cas9 genome editing method: current technologies and examples.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
27 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hybrid rice breeding offers an important strategy to improve rice production, in which the cultivation of a male sterile line is the key to the success of cross-breeding. CRISPR/Cas9 systems have been widely used in target-site genome editing, whereas their application for crop genetic improvement has been rarely reported. Here, using the CRISPR/Cas9 system, we induced specific mutations in TMS5, which is the most widely applied thermo-sensitive genic male sterility (TGMS) gene in China, and developed new "transgene clean" TGMS lines. We designed 10 target sites in the coding region of TMS5 for targeted mutagenesis using the CRISPR/Cas9 system and assessed the potential rates of on- and off-target effects. Finally, we established the most efficient construct, the TMS5ab construct, for breeding potentially applicable "transgene clean" TGMS lines. We also discussed factors that affect the editing efficiency according to the characteristics of different target sequences. Notably, using the TMS5ab construct, we developed 11 new "transgene clean" TGMS lines with potential applications in hybrid breeding within only one year in both rice subspecies. The application of our system not only significantly accelerates the breeding of sterile lines but also facilitates the exploitation of heterosis.
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