ArticleSensors (Basel, Switzerland)2008
Determination of Vitamin C (Ascorbic Acid) Using High Performance Liquid Chromatography Coupled with Electrochemical Detection.
Article in Sensors (Basel, Switzerland), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 164 citations in OpenAlex.
- A Green Approach to the Simultaneous Detection of Vitamin C, Paracetamol, and Caffeine Using an Aspartic Acid-Modified Waste Battery Electrode.ACS omega · 2026Article
- Design of a Nano-Functionalized Lab-on-Paper Optical Sensing Platform for On-Site Detection of Ascorbic Acid in Natural Fruit Juices.Applied biochemistry and biotechnology · 2025Article
- Development of a multi-source blue light irradiation platform for photometric quantification of ascorbic acidRSC advances · 2025Article
- Facile synthesis of ZnO/CuO/rGO nanohybrid for nonenzymatic electrochemical glucose sensor.RSC advances · 2025Article
- High-Performance Liquid Chromatography Quantification of Glyphosate, Aminomethylphosphonic Acid, and Ascorbate in Culture Medium and Microalgal Cells.Bio-protocol · 2025Article
- Understand the Stabilization Engineering of Ascorbic Acid, Mapping the Scheme for Stabilization, and Advancement.AAPS PharmSciTech · 2024Article
- A dual-signal optical sensing platform of CDs-MnORSC advances · 2024Article
- Nutritional and anti-nutritional compositional analysis of transgenic potatoes with late blight resistance.Frontiers in bioengineering and biotechnology · 2024Article
- A High-Performance Liquid Chromatography with Electrochemical Detection Method Developed for the Sensitive Determination of Ascorbic Acid: Validation, Application, and Comparison with Titration, Spectrophotometric, and High-Performance Liquid Chromatography with Diode-Array Detection Methods.Foods (Basel, Switzerland) · 2023Article
- Effect of Hydrogen Plasma Treatment on the Sensitivity of ZnO Based Electrochemical Non-Enzymatic Biosensor.Biosensors · 2023Article
- Article
- A novel wrinkled-leaf sesame mutant as a potential edible leafy vegetable rich in nutrients.Scientific reports · 2022Article
- Analysis of Phenolic Compounds in Food by Coulometric Array Detector: A Review.Sensors (Basel, Switzerland) · 2022Review
- Microwave induced thermally assisted solvent-based bonding of biodegradable thermoplastics: an eco-friendly rapid approach for fabrication of microfluidic devices and analyte detection.Scientific reports · 2022Article
- Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination.Nutrients · 2021Review
- Article
- Activity-Based Sensing of Ascorbate by Using Copper-Mediated Oxidative Bond Cleavage.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Microwave-assisted Synthesis of N,S-co-carbon Dots as Switch-on Fluorescent Sensor for Rapid and Sensitive Detection of Ascorbic Acid in Processed Fruit Juice.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2020Article
- Liquid Chromatography Analysis of Common Nutritional Components, in Feed and Food.Foods (Basel, Switzerland) · 2018Review
- Ultrasensitive determination of ascorbic acid by using cobalt oxyhydroxide nanosheets to enhance the chemiluminescence of the luminol-HRSC advances · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vitamin C (ascorbic acid, ascorbate, AA) is a water soluble organic compound that participates in many biological processes. The main aim of this paper was to utilize two electrochemical detectors (amperometric - Coulouchem III and coulometric - CoulArray) coupled with flow injection analysis for the detection of ascorbic acid. Primarily, we optimized the experimental conditions. The optimized conditions were as follows: detector potential 100 mV, temperature 25 °C, mobile phase 0.09% TFA:ACN, 3:97 (v/v) and flow rate 0.13 mL·min-1. The tangents of the calibration curves were 0.3788 for the coulometric method and 0.0136 for the amperometric one. The tangent of the calibration curve measured by the coulometric detector was almost 30 times higher than the tangent measured by the amperometric detector. Consequently, we coupled a CoulArray electrochemical detector with high performance liquid chromatography and estimated the detection limit for AA as 90 nM (450 fmol per 5 μL injection). The method was used for the determination of vitamin C in a pharmaceutical preparations (98 ± 2 mg per tablet), in oranges (Citrus aurantium) (varied from 30 to 56 mg/100 g fresh weight), in apples (Malus sp.) (varied from 11 to 19 mg/100 g fresh weight), and in human blood serum (varied from 38 to 78 μM). The recoveries were also determined.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.