ArticleScientific reports2016
Development of a rapid screen for the endodermal differentiation potential of human pluripotent stem cell lines.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- From Design to Single-Cell Cloning: A Complete RNP-Based CRISPR-Cas9 Protocol for Precision Gene Correction in Human iPSCs.Stem cell reviews and reports · 2026Article
- A transgene-free, human peri-gastrulation embryo model presents trilaminar embryonic disc-, amnion- and yolk sac-like structures.Nature cell biology · 2026Article
- Article
- Effect of hypoxia on aquaporins and hepatobiliary transport systems in human hepatic cells.Pediatric research · 2025Article
- Scalable production of tissue-like vascularized liver organoids from human PSCs.Experimental & molecular medicine · 2023Article
- The Exciting Realities and Possibilities of iPS-Derived Cardiomyocytes.Bioengineering (Basel, Switzerland) · 2023Review
- Comparing the mitochondrial signatures in ESCs and iPSCs and their neural derivations.Cell cycle (Georgetown, Tex.) · 2022Article
- Article
- Increased p53 signaling impairs neural differentiation in HUWE1-promoted intellectual disabilities.Cell reports. Medicine · 2021Article
- Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies.Analytical chemistry · 2021Article
- Distinct Mitochondrial Remodeling During Mesoderm Differentiation in a Human-Based Stem Cell Model.Frontiers in cell and developmental biology · 2021Article
- Three-Dimensional Imaging in Stem Cell-Based Researches.Frontiers in veterinary science · 2021Review
- A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates.Scientific reports · 2020Article
- Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations.EMBO molecular medicine · 2020Article
- Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells.International journal of molecular sciences · 2020Article
- Variable Outcomes in Neural Differentiation of Human PSCs Arise from Intrinsic Differences in Developmental Signaling Pathways.Cell reports · 2020Article
- Control of matrix stiffness promotes endodermal lineage specification by regulating SMAD2/3 via lncRNA LINC00458.Science advances · 2020Article
- Predicting differentiation potential of human pluripotent stem cells: Possibilities and challenges.World journal of stem cells · 2019Article
- Who Will Win: Induced Pluripotent Stem Cells Versus Embryonic Stem Cells for β Cell Replacement and Diabetes Disease Modeling?Current diabetes reports · 2018Review
- Directed reprogramming of comprehensively characterized dental pulp stem cells extracted from natal tooth.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A challenge facing the human pluripotent stem cell (hPSC) field is the variability observed in differentiation potential of hPSCs. Variability can lead to time consuming and costly optimisation to yield the cell type of interest. This is especially relevant for the differentiation of hPSCs towards the endodermal lineages. Endodermal cells have the potential to yield promising new knowledge and therapies for diseases affecting multiple organ systems, including lung, thymus, intestine, pancreas and liver, as well as applications in regenerative medicine and toxicology. Providing a means to rapidly, cheaply and efficiently assess the differentiation potential of multiple hPSCs is of great interest. To this end, we have developed a rapid small molecule based screen to assess the endodermal potential (EP) of hPSCs, based solely on definitive endoderm (DE) morphology. This drastically reduces the cost and time to identify lines suitable for use in deriving endodermal lineages. We demonstrate the efficacy of this screen using 10 different hPSCs, including 4 human embryonic stem cell lines (hESCs) and 6 human induced pluripotent stem cell lines (hiPSCs). The screen clearly revealed lines amenable to endodermal differentiation, and only lines that passed our morphological assessment were capable of further differentiation to hepatocyte like cells (HLCs).
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