ArticlePlant physiology2017
Two Membrane-Anchored Aspartic Proteases Contribute to Pollen and Ovule Development.
Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Four guidelines to identify bona fide protease-substrate pairs.Journal of experimental botany · 2025Review
- Identifying new players of gynoecium development using tissue-specific transcriptome data of Arabidopsis.Planta · 2025Article
- Genome-wide identification and expression patterns of the aspartic protease gene family in Epimedium pubescens.BMC genomics · 2025Article
- Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development.BMC plant biology · 2023Article
- Two aspartic proteases, BnaAP36s and BnaAP39s, regulate pollen tube guidance inMolecular breeding : new strategies in plant improvement · 2023Article
- Phylogenetic and AlphaFold predicted structure analyses provide insights for A1 aspartic protease family classification inFrontiers in plant science · 2023Article
- Article
- Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Plants (Basel, Switzerland) · 2022Article
- Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies.Frontiers in endocrinology · 2022Review
- Expression in Escherichia coli, Refolding, and Purification of Plant Aspartic Proteases.Methods in molecular biology (Clifton, N.J.) · 2022Article
- How Cysteine Protease GeneInternational journal of molecular sciences · 2021Article
- RNA-Seq analysis reveals potential regulators of programmed cell death and leaf remodelling in lace plant (Aponogeton madagascariensis).BMC plant biology · 2021Article
- Interaction between endogenous HPlant cell reports · 2021Article
- RNA Sequencing Reveals Rice Genes Involved in Male Reproductive Development under Temperature Alteration.Plants (Basel, Switzerland) · 2021Article
- Review
- Article
- Bridging the GAPs in plant reproduction: a comparison of plant and animal GPI-anchored proteins.Plant reproduction · 2020Review
- Genome-wide identification of the aspartic protease gene family and their response under powdery mildew stress in wheat.Molecular biology reports · 2020Article
- AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis.BMC plant biology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspartic proteases are a class of proteolytic enzymes with conserved aspartate residues, which are implicated in protein processing, maturation, and degradation. Compared with yeast and animals, plants possess a larger aspartic protease family. However, little is known about most of these enzymes. Here, we characterized two Arabidopsis (Arabidopsis thaliana) putative glycosylphosphatidylinositol (GPI)-anchored aspartic protease genes, A36 and A39, which are highly expressed in pollen and pollen tubes. a36 and a36 a39 mutants display significantly reduced pollen activity. Transmission electron microscopy and terminal-deoxynucleotidyl transferase-mediated nick end labeling assays further revealed that the unviable pollen in a36 a39 may undergo unanticipated apoptosis-like programmed cell death. The degeneration of female gametes also occurred in a36 a39 Aniline Blue staining, scanning electron microscopy, and semi in vitro guidance assays indicated that the micropylar guidance of pollen tubes is significantly compromised in a36 a39 A36 and A39 that were fused with green fluorescent protein are localized to the plasma membrane and display punctate cytosolic localization and colocalize with the GPI-anchored protein COBRA-LIKE10. Furthermore, in a36 a39, the abundance of highly methylesterified homogalacturonans and xyloglucans was increased significantly in the apical pollen tube wall. These results indicate that A36 and A39, two putative GPI-anchored aspartic proteases, play important roles in plant reproduction in Arabidopsis.
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