ArticleAntimicrobial agents and chemotherapy2017
Glycoside Hydrolases Degrade Polymicrobial Bacterial Biofilms in Wounds.
Article in Antimicrobial agents and chemotherapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
112 citing papers in PubMed, 2 syntheses or guidelines pooled it, 194 citations in OpenAlex.
- Novel biofilm-targeted therapeutics for oral infections: enzymes, EPS disruptors, phage/CRISPR, photodynamic and cold-plasma approaches - a systematic review.Frontiers in cellular and infection microbiology · 2026Pooled it
- Understanding bacterial biofilms: From definition to treatment strategies.Frontiers in cellular and infection microbiology · 2023Pooled it
- Bacteria-Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- The Oral Virome in Dental Caries: From Ecological Disruptors to Therapeutic Opportunities.Journal of microbiology and biotechnology · 2026Review
- Bimetallic-Node-Occupied MOF With Glycoside Hydrolase Activity for Efficient Bacterial Biofilm Hydrolysis.Angewandte Chemie (International ed. in English) · 2026Article
- Biochemical and Structural Characterization of Two-domain Glycoside Hydrolase PgaB fromACS infectious diseases · 2026Article
- Biofilm-Host Immune Crosstalk at the Diabetic Foot Ulcer Interface: Molecular Mechanisms, Immune Evasion, and Next-Generation Anti-Biofilm Strategies.Diabetes, metabolic syndrome and obesity : targets and therapy · 2026Review
- BacQuant: A Scalable Automated Image Processing Pipeline for Quantifying Biofilm Aggregates.microPublication biology · 2026Article
- Decoding the spatiotemporal heterogeneity of bacterial virulence gene expression using single-cell approaches.Frontiers in microbiology · 2026Review
- Rapid means of biofilm disruption induce the newly released (NRel) phenotype of enhanced antibiotic sensitivity.Frontiers in microbiology · 2026Article
- Article
- α-Amylase-Mediated Antibiotic Degradation and Sequestration inAntibiotics (Basel, Switzerland) · 2025Article
- Article
- Pseudomonas aeruginosa biofilm: treatment strategies to combat infection.Archives of microbiology · 2025Review
- Exploring marine-derived bioactive compounds for dual inhibition of Pseudomonas aeruginosa LpxA and LpxD: integrated bioinformatics and cheminformatics approaches.Molecular diversity · 2025Article
- Article
- Biofilm Dispersal and Wound Infection Clearance With Preclinical Debridement Agents.International wound journal · 2025Article
- Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components ofAntibiotics (Basel, Switzerland) · 2025Article
- Microplastics and antibiotic resistance genes as rising threats: Their interaction represents an urgent environmental concern.Current research in microbial sciences · 2025Review
- Structural characteristics, functions, and counteracting strategies of biofilms inComputational and structural biotechnology journal · 2025Review
52 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The persistent nature of chronic wounds leaves them highly susceptible to invasion by a variety of pathogens that have the ability to construct an extracellular polymeric substance (EPS). This EPS makes the bacterial population, or biofilm, up to 1,000-fold more antibiotic tolerant than planktonic cells and makes wound healing extremely difficult. Thus, compounds which have the ability to degrade biofilms, but not host tissue components, are highly sought after for clinical applications. In this study, we examined the efficacy of two glycoside hydrolases, α-amylase and cellulase, which break down complex polysaccharides, to effectively disrupt Staphylococcus aureus and Pseudomonas aeruginosa monoculture and coculture biofilms. We hypothesized that glycoside hydrolase therapy would significantly reduce EPS biomass and convert bacteria to their planktonic state, leaving them more susceptible to conventional antimicrobials. Treatment of S. aureus and P. aeruginosa biofilms, grown in vitro and in vivo, with solutions of α-amylase and cellulase resulted in significant reductions in biomass, dissolution of the biofilm, and an increase in the effectiveness of subsequent antibiotic treatments. These data suggest that glycoside hydrolase therapy represents a potential safe, effective, and new avenue of treatment for biofilm-related infections.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.