ArticleBMC nephrology2016
Quantitative characterization of T-cell repertoire and biomarkers in kidney transplant rejection.
Article in BMC nephrology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- Next generation sequencing based assessment of the alloreactive T cell receptor repertoire in kidney transplant patients during rejection: a prospective cohort study.BMC nephrology · 2019Trial
- Innate-immune crosstalk orchestrates T cell-mediated rejection in kidney transplants.Frontiers in immunology · 2026Article
- Editorial: Methods in alloimmunity and transplantation: 2025.Frontiers in immunology · 2026Article
- Unraveling CD8American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2025Review
- Discovering molecular signatures in kidney transplant biopsies with borderline changes and isolated V-lesions: single-cell RNA-sequencing analysis of human blood and tissue Spatial transcriptomics.Scientific reports · 2025Article
- Discrimination of Anti-Donor Response in Allogeneic Transplantation Using an Alloreactive T-Cell Detection Assay.Transplant international : official journal of the European Society for Organ Transplantation · 2025Article
- Pre-transplant T-cell clonal analysis identifies CD8Frontiers in immunology · 2025Article
- Editorial: Methods in alloimmunity and transplantation: 2023.Frontiers in immunology · 2024Article
- Single-cell transcriptomic analysis of renal allograft rejection reveals insights into intragraft TCR clonality.The Journal of clinical investigation · 2023Article
- Prospects of Cytomegalovirus-Specific T-Cell Receptors in Clinical Diagnosis and Therapy.Viruses · 2023Review
- Alloreactive T cells to Assess Acute Rejection Risk in Kidney Transplant Recipients.Transplantation direct · 2023Article
- T-Cell Receptor Repertoire Sequencing in the Era of Cancer Immunotherapy.Clinical cancer research : an official journal of the American Association for Cancer Research · 2023Article
- Clinical application of immune repertoire sequencing in solid organ transplant.Frontiers in immunology · 2023Review
- Article
- High-Throughput Sequencing of Complementarity Determining Region 3 in the Heavy Chain of B-Cell Receptor in Renal Transplant Recipients: A Preliminary Report.Journal of clinical medicine · 2022Article
- Modulation of tissue resident memory T cells by glucocorticoids after acute cellular rejection in lung transplantation.The Journal of experimental medicine · 2022Article
- Donor-derived cell-free DNA as a diagnostic tool in transplantation.Frontiers in genetics · 2022Review
- Perturbations of the T-cell immune repertoire in kidney transplant rejection.Frontiers in immunology · 2022Article
- Advances in single-cell sequencing: insights from organ transplantation.Military Medical Research · 2021Review
- The methods and advances of adaptive immune receptors repertoire sequencing.Theranostics · 2021Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundT-cell-mediated rejection (TCMR) remains a major cause of kidney allograft failure. The characterization of T-cell repertoire in different immunological disorders has emerged recently as a novel tool with significant implications. We herein sought to characterize T-cell repertoire using next generation sequencing to diagnose TCMR.
methodsIn this prospective study, we analyzed samples from 50 kidney transplant recipients. We collected blood and kidney transplant biopsy samples at sequential time points before and post transplant. We used next generation sequencing to characterize T-cell receptor (TCR) repertoire by using illumina miSeq on cDNA synthesized from RNA extracted from six patients' samples. We also measured RNA expression levels of FOXP3, CD8, CD4, granzyme and perforin in blood samples from all 50 patients.
resultsSeven patients developed TCMR during the first three months of the study. Out of six patients who had complete sets of blood and biopsy samples two had TCMR. We found an expansion of the TCR repertoire in blood at time of rejection when compared to that at pre-transplant or one-month post transplant. Patients with TCMR (n = 7) had significantly higher RNA expression levels of FOXP3, Perforin, Granzyme, CD4 and CD8 in blood samples than those with no TCMR (n = 43) (P = 0.02, P = 0.003, P = 0.002, P = 0.017, and P = 0.01, respectively).
conclusionsOur study provides a potential utilization of TCR clone kinetics analysis in the diagnosis of TCMR. This approach may allow for the identification of the expanded T-cell clones associated with the rejection and lead to potential noninvasive diagnosis and targeted therapies of TCMR.
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