ArticlePloS one2016
Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Biofilm Formation inMicroorganisms · 2026Article
- Immune interaction betweenFrontiers in cellular and infection microbiology · 2026Article
- Stenotrophomonas maltophilia complex: A broadly distributed emerging pathogen.PLoS pathogens · 2025Article
- Rethinking Aspergillosis in the Era of Microbiota and Mycobiota.Mycopathologia · 2024Review
- Bacteria and fungi of the lung: allies or enemies?Frontiers in pharmacology · 2024Review
- Interactions between Bacteria andJournal of fungi (Basel, Switzerland) · 2023Review
- The Fungal and Bacterial Interface in the Respiratory Mycobiome with a Focus onLife (Basel, Switzerland) · 2023Review
- Design and synthesis of eugenol/isoeugenol glycoconjugates and other analogues as antifungal agents againstRSC medicinal chemistry · 2022Article
- Persistent pleuritic chest pain in a patient with cystic fibrosis.Breathe (Sheffield, England) · 2022Article
- Clinical challenges treatingJAC-antimicrobial resistance · 2022Review
- Microdiversity ofEmerging microbes & infections · 2021Article
- Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction betweenJournal of fungi (Basel, Switzerland) · 2021Article
- Advances in the Microbiology of Stenotrophomonas maltophilia.Clinical microbiology reviews · 2021Article
- Review
- Allergic Diseases Caused byAntibiotics (Basel, Switzerland) · 2021Review
- Dual-purpose isocyanides produced byProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Effects of Tigecycline Combined with Azithromycin Against Biofilms of Multidrug-ResistantInfection and drug resistance · 2021Article
- Modulated Response ofFrontiers in cellular and infection microbiology · 2020Article
- Interactions betweenJournal of fungi (Basel, Switzerland) · 2019Review
- Time-Dependent Changes in Morphostructural Properties and Relative Abundances of Contributors inFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiofilms are communal structures of microorganisms that have long been associated with a variety of persistent infections poorly responding to conventional antibiotic or antifungal therapy. Aspergillus fumigatus fungus and Stenotrophomonas maltophilia bacteria are examples of the microorganisms that can coexist to form a biofilm especially in the respiratory tract of immunocompromised patients or cystic fibrosis patients. The aim of the present study was to develop and assess an in vitro model of a mixed biofilm associating S. maltophilia and A. fumigatus by using analytical and quantitative approaches. MATERIALS AND
methodsAn A. fumigatus strain (ATCC 13073) expressing a Green Fluorescent Protein (GFP) and an S. maltophilia strain (ATCC 13637) were used. Fungal and bacterial inocula (105 conidia/mL and 106 cells/mL, respectively) were simultaneously deposited to initiate the development of an in vitro mixed biofilm on polystyrene supports at 37°C for 24 h. The structure of the biofilm was analysed via qualitative microscopic techniques like scanning electron and transmission electron microscopy, and fluorescence microscopy, and by quantitative techniques including qPCR and crystal violet staining.
resultsAnalytic methods revealed typical structures of biofilm with production of an extracellular matrix (ECM) enclosing fungal hyphae and bacteria. Quantitative methods showed a decrease of A. fumigatus growth and ECM production in the mixed biofilm with antibiosis effect of the bacteria on the fungi seen as abortive hyphae, limited hyphal growth, fewer conidia, and thicker fungal cell walls.
conclusionFor the first time, a mixed A. fumigatus-S. maltophilia biofilm was validated by various analytical and quantitative approaches and the bacterial antibiosis effect on the fungus was demonstrated. The mixed biofilm model is an interesting experimentation field to evaluate efficiency of antimicrobial agents and to analyse the interactions between the biofilm and the airways epithelium.
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