ArticlePLoS genetics2016
Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- The human DBR1 interactome reveals coupling between intron lariat turnover, pre-mRNA splicing, and RNA quality control pathways.Cell stress & chaperones · 2026Article
- Clearance of splicing byproducts promotes plant defence.Nature plants · 2026Article
- RNA regulation in plants.Science China. Life sciences · 2026Review
- Circular RNA Vv-circCOR27 modulates thermotolerance through attenuating VvHSP90.2b-VvHsfA7a interaction in grapevine.The New phytologist · 2026Article
- Rice and shine: a review of miRNA-mediated responses to abiotic stress inFrontiers in plant science · 2026Review
- DBR1 orchestrates the fate of lariat RNA: debranching-dependent turnover and function.Nucleic acids research · 2025Review
- ebv-sisRNA-3 contributes to the formation of G4-associated R-loop upstream of EBV lytic replication origin in latently infected cells.Cell & bioscience · 2025Article
- Sequestration of DBR1 to stress granules promotes lariat intronic RNAs accumulation for heat-stress tolerance.Nature communications · 2024Article
- A repertoire of intronic lariat RNAs reveals tissue-specific regulation and target mimicry potential in plants.Science China. Life sciences · 2024Article
- Biomolecular condensates in plant RNA silencing: insights into formation, function, and stress responses.The Plant cell · 2024Review
- PlantIntronDB: a database for plant introns that host functional elements.Database : the journal of biological databases and curation · 2023Article
- Trichothiodystrophy-associated MPLKIP maintains DBR1 levels for proper lariat debranching and ectodermal differentiation.EMBO molecular medicine · 2023Article
- A founder DBR1 variant causes a lethal form of congenital ichthyosis.Human genetics · 2023Article
- microRNA biogenesis and stabilization in plants.Fundamental research · 2023Review
- Multi-omics of Circular RNAs and Their Responses to Hormones in Moso Bamboo (Phyllostachys edulis).Genomics, proteomics & bioinformatics · 2023Article
- A functional link between lariat debranching enzyme and the intron-binding complex is defective in non-photosensitive trichothiodystrophy.Molecular cell · 2023Article
- sic-4 Reports in sick! Loss of SICKLE induces salicylic acid-dependent cell death in Arabidopsis.Plant physiology · 2023Article
- Absence of SICKLE triggers programed cell death by disturbing alternative splicing and decay of mRNAs.Plant physiology · 2023Article
- The rich non-coding RNA landscape of the Drosophila antenna.Cell reports · 2023Article
- Do not panic: An intron-centric guide to alternative splicing.The Plant cell · 2023Review
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lariat RNAs formed as by-products of splicing are quickly degraded by the RNA debranching enzyme 1 (DBR1), leading to their turnover. Null dbr1 mutants in both animals and plants are embryo lethal, but the mechanism underlying the lethality remains unclear. Here we characterized a weak mutant allele of DBR1 in Arabidopsis, dbr1-2, and showed that a global increase in lariat RNAs was unexpectedly accompanied by a genome-wide reduction in miRNA accumulation. The dbr1-2 mutation had no effects on expression of miRNA biogenesis genes or primary miRNAs (pri-miRNAs), but the association of pri-miRNAs with the DCL1/HYL1 dicing complex was impaired. Lariat RNAs were associated with the DCL1/HYL1 dicing complex in vivo and competitively inhibited the binding of HYL1 with pri-miRNA. Consistent with the impacts of lariat RNAs on miRNA biogenesis, over-expression of lariat RNAs reduced miRNA accumulation. Lariat RNAs localized in nuclear bodies, and partially co-localize with HYL1, and both DCL1 and HYL1 were mis-localized in dbr1-2. Together with our findings that nearly four hundred lariat RNAs exist in wild type plants and that these lariat RNAs also associate with the DCL1/HYL1 dicing complex in vivo, we thus propose that lariat RNAs, as decoys, inhibit miRNA processing, suggesting a hitherto unknown layer of regulation in miRNA biogenesis.
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