ArticleLaboratory investigation; a journal of technical methods and pathology2017
Covalently deposited dyes: a new chromogen paradigm that facilitates analysis of multiple biomarkers in situ.
Article in Laboratory investigation; a journal of technical methods and pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Pentachroma O-H: A Five-Color Histological Staining Method for Enhanced Intestinal Tissue Analysis.International journal of molecular sciences · 2025Article
- Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens.Diagnostics (Basel, Switzerland) · 2025Article
- Digital analysis of the prostate tumor microenvironment with high-order chromogenic multiplexing.Journal of pathology informatics · 2024Article
- The stress response regulator HSF1 modulates natural killer cell anti-tumour immunity.Nature cell biology · 2024Article
- Article
- Microglia at sites of atrophy restrict the progression of retinal degeneration via galectin-3 and Trem2.The Journal of experimental medicine · 2024Article
- Quantitative multiplexed imaging technologies for single-cell analysis to assess predictive markers for immunotherapy in thoracic immuno-oncology: promises and challenges.British journal of cancer · 2023Review
- α2,6-Sialylation is Upregulated in Severe COVID-19 Implicating the Complement Cascade.medRxiv : the preprint server for health sciences · 2022Article
- Pulmonary Pathology of End-Stage COVID-19 Disease in Explanted Lungs and Outcomes After Lung Transplantation.American journal of clinical pathology · 2022Article
- Conventional histological and cytological staining with simultaneous immunohistochemistry enabled by invisible chromogens.Laboratory investigation; a journal of technical methods and pathology · 2022Article
- Evidence for continuity of interstitial spaces across tissue and organ boundaries in humans.Communications biology · 2021Article
- SITC cancer immunotherapy resource document: a compass in the land of biomarker discovery.Journal for immunotherapy of cancer · 2020Article
- Keratin 19 and mesenchymal markers for evaluation of epithelial-mesenchymal transition and stem cell niche components in primary biliary cholangitis by sequential elution-stripping multiplex immunohistochemistry.Journal of histotechnology · 2020Article
- Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy.Cancer communications (London, England) · 2020Review
- New Technologies to Image Tumors.Cancer treatment and research · 2020Article
- Enhancing the Value of Histopathological Assessment of Allograft Biopsy Monitoring.Transplantation · 2019Review
- Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies.Laboratory investigation; a journal of technical methods and pathology · 2017Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiplexed analysis of multiple biomarkers in a tissue sample requires use of reporter dyes with specific spectral properties that enable discrimination of signals. Conventional chromogens with broad absorbance spectra, widely used in immunohistochemistry (IHC), offer limited utility for multiplexed detection. Many dyes with narrow absorbance spectra, eg rhodamines, fluoresceins, and cyanines, potentially useful for multiplexed detection are well-characterized; however, generation of a chromogenic reagent useful for IHC analysis has not been demonstrated. Studies reported herein demonstrate utility of tyramine-chemistry for synthesis of a wide variety of new chromogenic dye conjugates useful for multiplexed in situ analysis using conventional light microscopes. The dyes, useful individually or in blends to generate new colors, provide signal sensitivity and dynamic range similar to conventional DAB chromogen, while enabling analysis of co-localized biomarkers. It is anticipated that this new paradigm will enable generation of a wide variety of new chromogens, useful for both research and clinical biomarker analysis that will benefit clinicians and patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.