ArticleScientific reports2016
Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- Tmx2 Maintains Mitochondrial Function to Support Preimplantation Embryogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Loss of function of ribosomal protein L13a blocks blastocyst formation and reveals a potential nuclear role in gene expression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- NCellular and molecular life sciences : CMLS · 2023Article
- Whole-genome transcriptome and DNA methylation dynamics of pre-implantation embryos reveal progression of embryonic genome activation in buffaloes.Journal of animal science and biotechnology · 2023Article
- Early embryonic lethality of mice lacking POLD2.Molecular reproduction and development · 2023Article
- Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation.Nature communications · 2022Article
- Berberine alleviates LPS-induced apoptosis, oxidation, and skewed lineages during mouse preimplantation development†.Biology of reproduction · 2022Article
- Surface functionalization of poly(dimethylsiloxane) substrates facilitates culture of pre-implantation mouse embryos by blocking non-selective adsorption.Journal of the Royal Society, Interface · 2022Article
- ZC3H4-a novel Cys-Cys-Cys-His-type zinc finger protein-is essential for early embryogenesis in mice†.Biology of reproduction · 2021Article
- Dynamic Oxygen Conditions Promote the Translocation of HIF-1BioMed research international · 2021Article
- Loss of RBBP4 results in defective inner cell mass, severe apoptosis, hyperacetylated histones and preimplantation lethality in mice†.Biology of reproduction · 2020Article
- Article
- MCRS1 is essential for epiblast development during early mouse embryogenesis.Reproduction (Cambridge, England) · 2020Article
- MED20 is essential for early embryogenesis and regulates NANOG expression.Reproduction (Cambridge, England) · 2019Article
- HIPPO signaling resolves embryonic cell fate conflicts during establishment of pluripotency in vivo.eLife · 2018Article
- Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts.Scientific reports · 2018Article
- Transcriptional profiles of crossbred embryos derived from yak oocytes in vitro fertilized with cattle sperm.Scientific reports · 2018Article
- Dynamics of the epigenetic landscape during the maternal-to-zygotic transition.Nature reviews. Molecular cell biology · 2018Review
- MouseEvoDevo · 2018Article
- Transcriptional Regulation and Genes Involved in First Lineage Specification During Preimplantation Development.Advances in anatomy, embryology, and cell biology · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
With readily available transcriptome-wide data, understanding the role of each expressed gene is an essential next step. Although RNAi technologies allow for genome-wide screens in cell culture, these approaches cannot replace strategies for discovery in the embryo. Here we present, for the first time, a knockdown screen in mouse preimplantation embryos. Early mammalian development encompasses dynamic cellular, molecular and epigenetic events that are largely conserved from mouse to man. We assayed 712 genes for requirements during preimplantation. We identified 59 genes required for successful development or outgrowth and implantation. We have characterized each phenotype and revealed cellular, molecular, and lineage specific defects following knockdown of transcript. Induced network analyses demonstrate this as a valid approach to identify networks of genes that play important roles during preimplantation. Our approach provides a robust and efficient strategy towards identification of novel phenotypes during mouse preimplantation and facilitates functional annotation of the mammalian transcriptome.
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Registered trials
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