ArticleScientific reports2016
Selection of reliable reference genes for normalization of quantitative RT-PCR from different developmental stages and tissues in amphioxus.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Evolutionary dynamics of sex determination in Branchiostoma belcheri driven by repeated transposition of a single novel gene.Nature communications · 2026Article
- Innovative GenExpA software for selecting suitable reference genes for reliable normalization of gene expression in melanoma.Scientific reports · 2022Article
- Evaluation of qPCR reference genes for taimen (Hucho taimen) under heat stress.Scientific reports · 2022Article
- Selection of reference genes for tissue/organ samples of adults of Eucryptorrhynchus scrobiculatus.PloS one · 2020Article
- Selection of Reference Genes for Quantitative Real-Time PCR inFrontiers in physiology · 2020Article
- Identification of stable reference genes for quantitative PCR in koalas.Scientific reports · 2018Article
- Genome-wide comparison of the protein-coding repertoire reveals fast evolution of immune-related genes in cephalochordates and Osteichthyes superclass.Oncotarget · 2018Article
- Rapid identification of Bactrocera zonata (Dip.: Tephritidae) using TaqMan real-time PCR assay.PloS one · 2018Article
- Selection and validation of suitable reference genes for qRT-PCR analysis in pear leaf tissues under distinct training systems.PloS one · 2018Article
- Genome-wide gene expression analysis in the amphioxus,Oncotarget · 2017Article
- Genome-wide gene expression analysis of amphioxus (Branchiostoma belcheri) following lipopolysaccharide challenge using strand-specific RNA-seq.RNA biology · 2017Article
- Selection of reference genes for qRT-PCR and expression analysis of high-altitude-related genes in grassland caterpillars (Lepidoptera: Erebidae:Ecology and evolution · 2017Article
- Reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus.Scientific reports · 2017Article
- Transcriptome-wide analysis of immune-responsive microRNAs against poly (I:C) challenge inOncotarget · 2017Article
- Reference gene selection to determine differences in mitochondrial gene expressions in phosphine-susceptible and phosphine-resistant strains of Cryptolestes ferrugineus, using qRT-PCR.Scientific reports · 2017Article
- Identification and validation of reference genes for gene expression analysis in Aphidius gifuensis (Hymenoptera: Aphidiidae).PloS one · 2017Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amphioxus is a closest living proxy to the ancestor of cephalochordates with vertebrates, and key animal for novel understanding in the evolutionary origin of vertebrate body plan, genome, tissues and immune system. Reliable analyses using quantitative real-time PCR (qRT-PCR) for answering these scientific questions is heavily dependent on reliable reference genes (RGs). In this study, we evaluated stability of thirteen candidate RGs in qRT-PCR for different developmental stages and tissues of amphioxus by four independent (geNorm, NormFinder, BestKeeper and deltaCt) and one comparative algorithms (RefFinder). The results showed that the top two stable RGs were the following: (1) S20 and 18 S in thirteen developmental stages, (2) EF1A and ACT in seven normal tissues, (3) S20 and L13 in both intestine and hepatic caecum challenged with lipopolysaccharide (LPS), and (4) S20 and EF1A in gill challenged with LPS. The expression profiles of two target genes (EYA and HHEX) in thirteen developmental stages were used to confirm the reliability of chosen RGs. This study identified optimal RGs that can be used to accurately measure gene expression under these conditions, which will benefit evolutionary and functional genomics studies in amphioxus.
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