Evidence map›Paper›PMID 27867707›Full record

ArticleBiomedical optics express2016

Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses.

Claudio Moretti, Andrea Antonini, Serena Bovetti, Carlo Liberale, Tommaso Fellin

Abstract read
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Scanless two-photon voltage imaging.Nature communications · 2024
    Article
  2. Article
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  5. Article
  6. Article
  7. Review
  8. Article
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  11. Towards circuit optogenetics.Current opinion in neurobiology · 2018
    Review
  12. Article
  13. Article
  14. Article
  15. Frontiers in aging neuroscience · 2018
    Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Claudio MorettiOptical Approaches to Brain Function Laboratory, Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
Andrea AntoniniOptical Approaches to Brain Function Laboratory, Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy; Nanostructures Department, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
Serena BovettiOptical Approaches to Brain Function Laboratory, Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
Carlo LiberaleNanostructures Department, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy; BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Tommaso FellinOptical Approaches to Brain Function Laboratory, Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.

Funding

The role of patterned activity in neuronal codes for behaviorU01NS090576 · NINDS · UNIVERSITY OF CHICAGO · PI FELLIN, TOMMASO, HISTED, MARK H · 2014 to 2016
$1.6M
NINDS NIH HHS U01 NS090576
6 · The paper itself

Abstract

Patterned illumination through the phase modulation of light is increasingly recognized as a powerful tool to investigate biological tissues in combination with two-photon excitation and light-sensitive molecules. However, to date two-photon patterned illumination has only been coupled to traditional microscope objectives, thus limiting the applicability of these methods to superficial biological structures. Here, we show that phase modulation can be used to efficiently project complex two-photon light patterns, including arrays of points and large shapes, in the focal plane of graded index (GRIN) lenses. Moreover, using this approach in combination with the genetically encoded calcium indicator GCaMP6, we validate our system performing scanless functional imaging in rodent hippocampal networks

Indexed as

(120.5060) Phase modulation(170.2150) Endoscopic imaging(170.2520) Fluorescence microscopy(180.4315) Nonlinear microscopy

Identifiers

PMID27867707
PMCPMC5102538

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.