ArticlePlant molecular biology2017
Transgenic citrus expressing synthesized cecropin B genes in the phloem exhibits decreased susceptibility to Huanglongbing.
Article in Plant molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 113 citations in OpenAlex.
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- 'Candidatus Liberibacter Asiaticus' Effector SECP8 Subverts Salicylic Acid-Mediated Citrus Immunity via a Two-Pronged Impairment of CsTCP15 Dimerization.Plant biotechnology journal · 2026Article
- Antimicrobial peptides: An important link in the game theory between plants and pathogens.Journal of advanced research · 2026Review
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- Pangenomic Approach for the Identification and Functional Characterization of Active GASA Antimicrobial Genes in Citrus Rootstocks for Resistance Breeding Against Bacterial Pathogens.Plants (Basel, Switzerland) · 2026Article
- A secretory protein from "Autophagy · 2025Article
- Microwave Treatment for Citrus Huanglongbing Control: Pathogen Elimination and Metabolomic Analysis.Plants (Basel, Switzerland) · 2025Article
- Recent progress in the understanding of Citrus Huanglongbing: from the perspective of pathogen and citrus host.Molecular breeding : new strategies in plant improvement · 2024Article
- Innovative strategies for characterizing and managing huanglongbing in citrus.World journal of microbiology & biotechnology · 2024Review
- Effector CLas0185 targets methionine sulphoxide reductase B1 of Citrus sinensis to promote multiplication of 'Candidatus Liberibacter asiaticus' via enhancing enzymatic activity of ascorbate peroxidase 1.Molecular plant pathology · 2024Article
- Citrus transcription factor CsERF1 is involved in the response to citrus tristeza disease.Frontiers in plant science · 2024Article
- Overexpressing CsSABP2 enhances tolerance to Huanglongbing and citrus canker inFrontiers in plant science · 2024Article
- The interaction between the lemon ribosomal protein ClRPS9-2 and citrus yellow vein clearing virus coat protein affects viral infection and gene silencing suppressor activity.Molecular plant pathology · 2023Article
- An endolysin gene fromHorticulture research · 2023Article
- Unleashing the Potential of EIL Transcription Factors in Enhancing Sweet Orange Resistance to Bacterial Pathologies: Genome-Wide Identification and Expression Profiling.International journal of molecular sciences · 2023Article
- Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of "Candidatus Liberibacter asiaticus" in citrus.Molecular horticulture · 2023Article
- Genome-wide identification and comparative expression profiling of the WRKY transcription factor family in two Citrus species with different Candidatus Liberibacter asiaticus susceptibility.BMC plant biology · 2023Article
- Highly efficientFrontiers in plant science · 2023Article
- Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies.Plants (Basel, Switzerland) · 2022Review
- Overexpression of the salicylic acid binding protein 2 (SABP2) from tobacco enhances tolerance against Huanglongbing in transgenic citrus.Plant cell reports · 2022Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageExpression of synthesized cecropin B genes in the citrus phloem, where Candidatus Liberibacter asiaticus resides, significantly decreased host susceptibility to Huanglongbing. Huanglongbing (HLB), associated with Candidatus Liberibacter asiaticus bacteria, is the most destructive disease of citrus worldwide. All of the commercial sweet orange cultivars lack resistance to this disease. The cationic lytic peptide cecropin B, isolated from the Chinese tasar moth (Antheraea pernyi), has been shown to effectively eliminate bacteria. In this study, we demonstrated that transgenic citrus (Citrus sinensis Osbeck) expressing the cecropin B gene specifically in the phloem had a decreased susceptibility to HLB. Three plant codon-optimized synthetic cecropin B genes, which were designed to secrete the cecropin B peptide into three specific sites, the extracellular space, the cytoplasm, and the endoplasmic reticulum, were constructed. Under the control of the selected phloem-specific promoter GRP1.8, these constructs were transferred into the citrus genome. All of the cecropin B genes were efficiently expressed in the phloem of transgenic plants. Over more than a year of evaluation, the transgenic lines exhibited reduced disease severity. Bacterial populations in transgenic lines were significantly lower than in the controls. Two lines, in which bacterial populations were significantly lower than in others, showed no visible symptoms. Thus, we demonstrated the potential application of the phloem-specific expression of an antimicrobial peptide gene to protect citrus plants from HLB.
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