Evidence map›Paper›PMID 27865632›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil.

Pedro Paulo Feitosa de Albuquerque, André de Souza Santos, Orestes Luiz de Souza Neto, Pomy de Cássia Peixoto Kim, Erika Fernanda Torres Samico Fernandes Cavalcanti, Júnior Mário Baltazar de Oliveira, Rinaldo Aparecido Mota, José Wilton Pinheiro Júnior

Abstract read
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Identification ofMicroorganisms · 2020
    Article
  4. Development of Rapid Extraction Method ofDiagnostics (Basel, Switzerland) · 2019
    Article
  5. Exploring the potential hazard ofVeterinary world · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pedro Paulo Feitosa de AlbuquerqueUniversidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil. Electronic address: ppfalbuquerque@gmail.com.
André de Souza SantosUniversidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
Orestes Luiz de Souza NetoUniversidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
Pomy de Cássia Peixoto KimUniversidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
Erika Fernanda Torres Samico Fernandes CavalcantiUniversidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
Júnior Mário Baltazar de OliveiraUniversidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
Rinaldo Aparecido MotaUniversidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
José Wilton Pinheiro JúniorUniversidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was found in bovine milk samples from the State of Pernambuco. To the best of our knowledge, this is the first report of MAP DNA found in bovine milk in Northeast Brazil. We also demonstrated the qPCR technique is more sensitive than conventional PCR with respect to detection of MAP in milk samples.

Indexed as

Food MicrobiologyAnimalsBrazilCattleDNA, BacterialMilkMycobacterium avium subsp. paratuberculosisParatuberculosisPolymerase Chain ReactionSensitivity and SpecificityDNA, BacterialDiagnosisMilkMolecular biologyParatuberculosis

Identifiers

PMID27865632
PMCPMC5221396

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.