ArticleProceedings of the National Academy of Sciences of the United States of America2016
Construction of a male sterility system for hybrid rice breeding and seed production using a nuclear male sterility gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 142 papers.
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Who cites it
142 citing papers in PubMed, 273 citations in OpenAlex.
- The SbTDR gene is required for anther development and male fertility in Sorghum bicolor.Plant reproduction · 2026Article
- Fine mapping and candidate gene analysis of tassel glume closure1 (tgc1) gene in maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Innovations in hybrid rice seed production: Integrating agronomy, mechanization, and biotechnology.Plant communications · 2026Review
- Intelligent GMS seed production and breeding system in Medicago sativa.Science China. Life sciences · 2026Article
- The receptor-like pseudokinase LENG stimulates chilling tolerance in rice by inhibiting the activity of the OsCRPK1-OsGF14d module.Journal of integrative plant biology · 2026Article
- TaRIP2 Positively Regulates Wheat Pollen Wall Formation Through MYB80-Controlled Lipid Metabolism.Plant biotechnology journal · 2026Article
- Developing an Efficient System for Hybrid Rice Seed Production Using Partial Male Sterility.Plant biotechnology journal · 2026Article
- An all-in-one visual selection system for male-sterile line production in maize and rice.Plant communications · 2026Article
- Unraveling Advances in Rice Male Sterility Systems: From Genetic Basis to Hybrid Breeding Innovation.Plants (Basel, Switzerland) · 2026Review
- Mutation in HER1 enhances stigma exsertion and hybrid seed production in rice.Nature communications · 2026Article
- Advances in the Mechanistic Study of Environment-Sensitive Genic Male Sterility in Rice.Rice (New York, N.Y.) · 2026Review
- Advancements in genomic crop techniques and considerations for regulation and food safety.Transgenic research · 2025Review
- Transgene-Killer-CRISPR version 2 (TKC2) eliminates occasional transgene escape by coupling with a RUBY reporter.Plant biotechnology journal · 2025Article
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- Exploring the Strategies of Male Sterility for Hybrid Development in Hexaploid Wheat: Prevailing Methods and Potential Approaches.Rice (New York, N.Y.) · 2025Review
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
- Agricultural biotechnology in China: product development, commercialization, and perspectives.aBIOTECH · 2025Review
- Development of a semi-dwarf dominant genic male sterile wheat with seed red fluorescence as a visible marker.Molecular breeding : new strategies in plant improvement · 2025Article
82 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The breeding and large-scale adoption of hybrid seeds is an important achievement in agriculture. Rice hybrid seed production uses cytoplasmic male sterile lines or photoperiod/thermo-sensitive genic male sterile lines (PTGMS) as female parent. Cytoplasmic male sterile lines are propagated via cross-pollination by corresponding maintainer lines, whereas PTGMS lines are propagated via self-pollination under environmental conditions restoring male fertility. Despite huge successes, both systems have their intrinsic drawbacks. Here, we constructed a rice male sterility system using a nuclear gene named Oryza sativa No Pollen 1 (OsNP1). OsNP1 encodes a putative glucose-methanol-choline oxidoreductase regulating tapetum degeneration and pollen exine formation; it is specifically expressed in the tapetum and miscrospores. The osnp1 mutant plant displays normal vegetative growth but complete male sterility insensitive to environmental conditions. OsNP1 was coupled with an α-amylase gene to devitalize transgenic pollen and the red fluorescence protein (DsRed) gene to mark transgenic seed and transformed into the osnp1 mutant. Self-pollination of the transgenic plant carrying a single hemizygous transgene produced nontransgenic male sterile and transgenic fertile seeds in 1:1 ratio that can be sorted out based on the red fluorescence coded by DsRed Cross-pollination of the fertile transgenic plants to the nontransgenic male sterile plants propagated the male sterile seeds of high purity. The male sterile line was crossed with ∼1,200 individual rice germplasms available. Approximately 85% of the F1s outperformed their parents in per plant yield, and 10% out-yielded the best local cultivars, indicating that the technology is promising in hybrid rice breeding and production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.