ArticleProceedings of the National Academy of Sciences of the United States of America2016
Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers, 1 of them a synthesis that pooled it.
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Who cites it
145 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Brassicaceae Family Displays Divergent, Shoot-Skewed NLR Resistance Gene Expression.Plant physiology · 2018Pooled it
- Assessment of the Effectiveness of Probiotics-assisted Physical Interventions in the Management of Chronic Periodontitis: A Randomized Controlled Clinical Trial.Probiotics and antimicrobial proteins · 2025Trial
- Evaluating the Role of Postbiotics in the Modulation of Human Oral Microbiota: A Randomized Controlled Clinical Trial.Probiotics and antimicrobial proteins · 2025Trial
- Functional capacities drive recruitment of bacteria into plant root microbiota.Nature microbiology · 2026Article
- Integrated multi-omics decipher the complex nodule microbiota and distinct Frankiaceae symbiotic traits in wild actinorhizal plants.The New phytologist · 2026Article
- Impact of pectin methylesterase on endophyte communities and pathogen resistance in Arabidopsis under heat stress.World journal of microbiology & biotechnology · 2026Article
- Nodule-Microbiome Dynamics: Deciphering the Complexities of Nodule Symbiosis and the Root Microbiome.International journal of molecular sciences · 2026Review
- Fruit function beyond dispersal: effect of fruit decomposition on the plant microbiome assembly.The New phytologist · 2026Article
- Fast track to environmentally adapted rhizobia for growing soybean at northern latitudes using citizen science.The ISME journal · 2026Article
- The impact of dinotefuran application at different concentrations on soil microbial communities in vineyards.Frontiers in microbiology · 2026Article
- Effects of biochar-based amendments on enzyme activity, microbial biomass, and bacterial communities in mildly cadmium-contaminated paddy soils.Frontiers in microbiology · 2026Article
- Loss of the flagellar regulator FlhC changes the transcriptional response of plant-associatedISME communications · 2026Article
- Effects of Autoinducer-2 on rumen bacterial community succession during in vitro fermentation with oat andFrontiers in microbiology · 2026Article
- Article
- Microbial drivers of root plasticity.The New phytologist · 2025Review
- Dual-Action Grouper Bone and Wakame Hydrolysates Supplement Enhances Exercise Performance and Modulates Gut Microbiota in Mice.Nutrients · 2025Article
- Seed-Derived Synthetic Microbial Communities (SynComs) fromMicroorganisms · 2025Article
- Pre-Crop Choice Shapes Nematode-Attached Bacterial Communities Associated With Reduced Pratylenchus penetrans Invasion of Barley Roots.Environmental microbiology · 2025Article
- Soybean nodulation shapes the rhizosphere microbiome to increase rapeseed yield.Journal of advanced research · 2025Article
- Roles of oral and gut microbiota in acute myocardial infarction.Journal of advanced research · 2025Article
85 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lotus japonicus has been used for decades as a model legume to study the establishment of binary symbiotic relationships with nitrogen-fixing rhizobia that trigger root nodule organogenesis for bacterial accommodation. Using community profiling of 16S rRNA gene amplicons, we reveal that in Lotus, distinctive nodule- and root-inhabiting communities are established by parallel, rather than consecutive, selection of bacteria from the rhizosphere and root compartments. Comparative analyses of wild-type (WT) and symbiotic mutants in Nod factor receptor5 (nfr5), Nodule inception (nin) and Lotus histidine kinase1 (lhk1) genes identified a previously unsuspected role of the nodulation pathway in the establishment of different bacterial assemblages in the root and rhizosphere. We found that the loss of nitrogen-fixing symbiosis dramatically alters community structure in the latter two compartments, affecting at least 14 bacterial orders. The differential plant growth phenotypes seen between WT and the symbiotic mutants in nonsupplemented soil were retained under nitrogen-supplemented conditions that blocked the formation of functional nodules in WT, whereas the symbiosis-impaired mutants maintain an altered community structure in the nitrogen-supplemented soil. This finding provides strong evidence that the root-associated community shift in the symbiotic mutants is a direct consequence of the disabled symbiosis pathway rather than an indirect effect resulting from abolished symbiotic nitrogen fixation. Our findings imply a role of the legume host in selecting a broad taxonomic range of root-associated bacteria that, in addition to rhizobia, likely contribute to plant growth and ecological performance.
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