Evidence map›Paper›PMID 27863553›Full record

ArticleVeterinary immunology and immunopathology2016

A comparison of serum and plasma cytokine values using a multiplexed assay in cats.

Margaret E Gruen, Kristen M Messenger, Andrea E Thomson, Emily H Griffith, Hayley Paradise, Shelly Vaden, B D X Lascelles

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary immunology and immunopathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Margaret E GruenComparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA; Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: megruen@ncsu.edu.
Kristen M MessengerComparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA; Department of Molecular and Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, Raleigh, NC, USA. Electronic address: kmmessen@ncsu.edu.
Andrea E ThomsonComparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA. Electronic address: aethomso@ncsu.edu.
Emily H GriffithDepartment of Statistics, North Carolina State University, 2311 Stinson Drive, Raleigh, NC 27695, USA. Electronic address: eghohmei@ncsu.edu.
Hayley ParadiseComparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA.
Shelly VadenDepartment of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, Raleigh, NC, USA. Electronic address: slvaden@ncsu.edu.
B D X LascellesComparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA; Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA; Center for Pain Research and Innovation, University of North Carolina School of Dentistry, Chapel Hill, NC 27599, USA. Electronic address: dxlascel@ncsu.edu.
North Carolina State University · USUniversity of North Carolina at Chapel Hill · US

Funding

Comparative Medicine and Translational Research Training ProgramT32OD011130 · OD · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI Lauren Virginia Schnabel · 2012 to 2026
$7.8M
NIH HHS T32 OD011130
6 · The paper itself

Abstract

backgroundDegenerative joint disease (DJD) is highly prevalent in cats, and pain contributes to morbidity. In humans, alterations of cytokine concentrations have been associated with joint deterioration and pain. Similar changes have not been investigated in cats. Cytokine concentrations can be measured using multiplex technology with small samples of serum or plasma, however, serum and plasma are not interchangeable for most bioassays. Correlations for cytokine concentrations between serum and plasma have not been evaluated in cats.

objectiveTo evaluate the levels of detection and agreement between serum and plasma samples in cats. ANIMALS: Paired serum and plasma samples obtained from 38 cats.

methodsBlood was collected into anti-coagulant free and EDTA Vacutainer

resultsAgreement between serum and plasma for many analytes was high, however correlation coefficients ranged from -0.01 to 0.97. Results from >50% of samples were below the lower limit of quantification for both serum and plasma for nine analytes, and for an additional three analytes for plasma only. CONCLUSIONS AND CLINICAL IMPORTANCE: While serum and plasma agreement was generally good, detection was improved using serum samples.

Indexed as

AnimalsBiomarkersBlood Chemical AnalysisCatsCytokinesImmunoassayPlasmaBiomarkersCytokinesBiomarkerFelineImmunoassay

Identifiers

PMID27863553
PMCPMC5522725
OpenAlexW2537752074

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.