ArticleVeterinary immunology and immunopathology2016
Optimisation and validation of a PCR for antigen receptor rearrangement (PARR) assay to detect clonality in canine lymphoid malignancies.
Article in Veterinary immunology and immunopathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Correlation between clinical variables and overall survival time in dogs with multicentric B-cell lymphoma: a retrospective study in Taiwan.Frontiers in veterinary science · 2026Article
- Diagnostic Sensitivity of the PCR for Antigen Receptor Rearrangement (PARR) Assay for Canine Plasma Cell Tumors.Veterinary clinical pathology · 2025Article
- Prognostic Utility of the Flow Cytometry and Clonality Analysis Results for Feline Lymphomas.Veterinary sciences · 2024Article
- Review
- Epigenetic Alterations in Canine Malignant Lymphoma: Future and Clinical Outcomes.Animals : an open access journal from MDPI · 2023Review
- Co-Expression of T- and B-Cell Markers in a Canine Intestinal Lymphoma: A Case Report.Animals : an open access journal from MDPI · 2022Article
- Doing more with less: multiple uses of a single slide in veterinary cytology. A practical approach.Veterinary research communications · 2022Review
- Feline and Canine Cutaneous Lymphocytosis: Reactive Process or Indolent Neoplastic Disease?Veterinary sciences · 2022Article
- Progressive gammopathy and coagulopathy in a young English bulldog.The Canadian veterinary journal = La revue veterinaire canadienne · 2021Article
- Differentiation of lymphocytic-plasmacytic enteropathy and small cell lymphoma in cats using histology-guided mass spectrometry.Journal of veterinary internal medicine · 2020Article
- Plasma cell leukemia with plasmablastic morphology in a dog.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Polymerase chain reaction for antigen receptor rearrangement: Benchmarking performance of a lymphoid clonality assay in diverse canine sample types.Journal of veterinary internal medicine · 2019Article
- Clonality testing in the lymph nodes from dogs with lymphadenomegaly due to Leishmania infantum infection.PloS one · 2019Article
- The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma.Oncotarget · 2017Article
- Dogs with Acute Myeloid Leukemia Have Clonal Rearrangements in T and B Cell Receptors.Frontiers in veterinary science · 2017Article
- Clonal T Cell Proliferation Induced by Acute Anaplasmosis in a Dog.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
PCR for antigen receptor gene rearrangements (PARR) analysis is being increasingly used to assist diagnosis of canine lymphoma. In this study, PARR was carried out on consecutive samples received as part of routine diagnostic practice from 271 patients: 195 with lymphoid malignancies, 53 with reactive conditions and 23 with other neoplasms. Initially, published primer sets were used but later minor primer modifications were introduced and primers were rationalised to give a PARR panel that provides a good compromise between sensitivity and cost. Results were compared to diagnoses made by histology or cytology, coupled with immunophenotyping by flow cytometry or immunohistochemistry where possible. After exclusion of 11 poor quality samples, 230/260 (88%) gave a clear result with 162/163 (99%) of samples classified as clonal and 56/67 (84%) classified as polyclonal giving results concordant with the cytological/histological diagnosis. Among 30 samples with equivocal results, 21 had clonal peaks in a polyclonal background and nine showed little amplification. These were from patients with a range of neoplastic and non-neoplastic conditions emphasising the need to interpret such results carefully in concert with other diagnostic tests. The combination of primer sets used in this study resulted in a robust, highly specific and sensitive assay for detecting clonality.
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