Evidence map›Paper›PMID 27863537›Full record

ArticleJournal of extracellular vesicles2016

High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources.

Reka A Haraszti, Marie-Cecile Didiot, Ellen Sapp, John Leszyk, Scott A Shaffer, Hannah E Rockwell, Fei Gao, Niven R Narain, Marian DiFiglia, Michael A Kiebish and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 432 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
432citing papers in PubMed, 1 pooled it
27.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

432 citing papers in PubMed, 1 synthesis or guideline pooled it, 733 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Review
  5. Extracellular Vesicles and Their Role in Osteogenesis.Bioengineering (Basel, Switzerland) · 2026
    Review
  6. Biology and Therapeutic Potential of Exosomes, Targeted Drug Delivery.International journal of molecular sciences · 2026
    Review
  7. Review
  8. Article
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  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
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  17. Review
  18. Article
  19. Article
  20. Article

372 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Reka A HarasztiRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA.
Marie-Cecile DidiotRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA.
Ellen SappMassGeneral Institute for Neurodegenerative Disease, Charlestown, MA, USA.
John LeszykProteomics and Mass Spectrometry Facility, University of Massachusetts Medical School, Worcester, MA, USA.
Scott A ShafferBiochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA.
Hannah E RockwellBerg LLC, Framingham, MA, USA.
Fei GaoBerg LLC, Framingham, MA, USA.
Niven R NarainBerg LLC, Framingham, MA, USA.
Marian DiFigliaMassGeneral Institute for Neurodegenerative Disease, Charlestown, MA, USA.
Michael A KiebishBerg LLC, Framingham, MA, USA.
Neil AroninRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA.
Anastasia KhvorovaRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA.
University of Massachusetts Chan Medical School · USFramingham State University · USMaineGeneral Medical Center · USMassachusetts General Hospital · US

Funding

Exosome based therapeutics in Huntington's diseaseUH3TR000888 · NCATS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI ARONIN, NEIL, KHVOROVA, ANASTASIA · 2015 to 2017
$3.0M
Inter and intra-cellular trafficking pathways for natural and therapeutic RNAsR01GM108803 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI KHVOROVA, ANASTASIA · 2014 to 2018
$2.0M
Exosome based therapeutics in Huntington's diseaseUH2TR000888 · NCATS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI ARONIN, NEIL · 2013 to 2014
$1.1M
NCATS NIH HHS UH2 TR000888NCATS NIH HHS UH3 TR000888NIGMS NIH HHS R01 GM108803
6 · The paper itself

Abstract

Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for use in diagnostics, therapeutics and drug delivery. However, little is known about the relationship of protein and lipid composition of EVs and their source cells. Here, we report high-resolution lipidomic and proteomic analyses of exosomes and MVs derived by differential ultracentrifugation from 3 different cell types: U87 glioblastoma cells, Huh7 hepatocellular carcinoma cells and human bone marrow-derived mesenchymal stem cells (MSCs). We identified 3,532 proteins and 1,961 lipid species in the screen. Exosomes differed from MVs in several different areas: (a) The protein patterns of exosomes were more likely different from their cells of origin than were the protein patterns of MVs; (b) The proteomes of U87 and Huh7 exosomes were similar to each other but different from the proteomes of MSC exosomes, whereas the lipidomes of Huh7 and MSC exosomes were similar to each other but different from the lipidomes of U87 exosomes; (c) exosomes exhibited proteins of extracellular matrix, heparin-binding, receptors, immune response and cell adhesion functions, whereas MVs were enriched in endoplasmic reticulum, proteasome and mitochondrial proteins. Exosomes and MVs also differed in their types of lipid contents. Enrichment in glycolipids and free fatty acids characterized exosomes, whereas enrichment in ceramides and sphingomyelins characterized MVs. Furthermore, Huh7 and MSC exosomes were specifically enriched in cardiolipins; U87 exosomes were enriched in sphingomyelins. This study comprehensively analyses the protein and lipid composition of exosomes, MVs and source cells in 3 different cell types.

Indexed as

cancerextracellular vesicleslipidomicsproteomicsstem cells

Identifiers

PMID27863537
PMCPMC5116062
OpenAlexW2553886049

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.