ArticleJournal of tissue engineering and regenerative medicine2018
Scaffold-free tissue engineering of functional corneal stromal tissue.
Article in Journal of tissue engineering and regenerative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Advances in Corneal Tissue Engineering: Comparative Performance of Bioengineered Grafts in Animal Models.Medicina (Kaunas, Lithuania) · 2025Pooled it
- Advanced bioengineering strategies broaden the therapeutic landscape for corneal failure.Frontiers in bioengineering and biotechnology · 2024Review
- Posterior corneoscleral limbus: Architecture, stem cells, and clinical implications.Progress in retinal and eye research · 2023Review
- Tissue Engineered Mini-Cornea Model for Eye Irritation Test.Tissue engineering and regenerative medicine · 2023Article
- Editorial: Regenerating the dentin-pulp complex: understanding the challenges that lie ahead.Frontiers in bioengineering and biotechnology · 2023Article
- Regenerative therapy for the Cornea.Progress in retinal and eye research · 2022Review
- Promoting and Orienting Axon Extension Using Scaffold-Free Dental Pulp Stem Cell Sheets.ACS biomaterials science & engineering · 2022Article
- Dental Pulp Cell Sheets Enhance Facial Nerve Regeneration via Local Neurotrophic Factor Delivery.Tissue engineering. Part A · 2021Article
- Induction of Irritation and Inflammation in a 3D Innervated Tissue Model of the Human Cornea.ACS biomaterials science & engineering · 2020Article
- The anti-scarring effect of corneal stromal stem cell therapy is mediated by transforming growth factor β3.Eye and vision (London, England) · 2020Article
- Bioengineering Approaches for Corneal Regenerative Medicine.Tissue engineering and regenerative medicine · 2020Review
- Corneal stromal stem cells restore transparency after NStem cells translational medicine · 2020Article
- Engineering topography: Effects on corneal cell behavior and integration into corneal tissue engineering.Bioactive materials · 2019Review
- Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle-Mediated Delivery of miRNA.Stem cells translational medicine · 2019Article
- Assembly and Application of a Three-Dimensional Human Corneal Tissue Model.Current protocols in toxicology · 2019Article
- Graphene-based 3D scaffolds in tissue engineering: fabrication, applications, and future scope in liver tissue engineering.International journal of nanomedicine · 2019Review
- Corneal Repair and Regeneration: Current Concepts and Future Directions.Frontiers in bioengineering and biotechnology · 2019Review
- Stemness and Regenerative Potential of Corneal Stromal Stem Cells and Their Secretome After Long-Term Storage: Implications for Ocular Regeneration.Investigative ophthalmology & visual science · 2018Article
- Regenerating Eye Tissues to Preserve and Restore Vision.Cell stem cell · 2018Review
- Substance P and patterned silk biomaterial stimulate periodontal ligament stem cells to form corneal stroma in a bioengineered three-dimensional model.Stem cell research & therapy · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Blinding corneal scarring is predominately treated with allogeneic graft tissue; however, there is a worldwide shortage of donor tissue leaving millions in need of therapy. Human corneal stromal stem cells (CSSC) have been shown produce corneal tissue when cultured on nanofibre scaffolding, but this tissue cannot be readily separated from the scaffold. In this study, scaffold-free tissue engineering methods were used to generate biomimetic corneal stromal tissue constructs that can be transplanted in vivo without introducing the additional variables associated with exogenous scaffolding. CSSC were cultured on substrates with aligned microgrooves, which directed parallel cell alignment and matrix organization, similar to the organization of native corneal stromal lamella. CSSC produced sufficient matrix to allow manual separation of a tissue sheet from the grooved substrate. These constructs were cellular and collagenous tissue sheets, approximately 4 μm thick and contained extracellular matrix molecules typical of corneal tissue including collagen types I and V and keratocan. Similar to the native corneal stroma, the engineered corneal tissues contained long parallel collagen fibrils with uniform diameter. After being transplanted into mouse corneal stromal pockets, the engineered corneal stromal tissues became transparent, and the human CSSCs continued to express human corneal stromal matrix molecules. Both in vitro and in vivo, these scaffold-free engineered constructs emulated stromal lamellae of native corneal stromal tissues. Scaffold-free engineered corneal stromal constructs represent a novel, potentially autologous, cell-generated, biomaterial with the potential for treating corneal blindness. Copyright © 2016 John Wiley & Sons, Ltd.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.