ArticlePlant biotechnology journal2017
Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing.
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
127 citing papers in PubMed, 1 synthesis or guideline pooled it, 344 citations in OpenAlex.
- Which Factors Affect the Occurrence of Off-Target Effects Caused by the Use of CRISPR/Cas: A Systematic Review in Plants.Frontiers in plant science · 2020Pooled it
- Genome Editing Approaches in Flax (International journal of molecular sciences · 2026Review
- Effects of CRISPR technology on agricultural sustainability: global applications and turkish perspective.Transgenic research · 2026Review
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- Targeted mutagenesis ofaBIOTECH · 2025Article
- Article
- Complexity Meets Risk-The Next Generation of Genome-Edited Plants Challenges Established Concepts for Environmental Risk Assessment in the EU.Plants (Basel, Switzerland) · 2025Review
- Engineering DMNT emission in cotton enhances direct and indirect defense against mirid bugs.Journal of advanced research · 2025Article
- Direct and indirect effects of multiplex genome editing of F5H and FAD2 in oil crop camelina.Plant biotechnology journal · 2025Article
- Born of frustration: the emergence of Camelina sativa as a platform for lipid biotechnology.Plant physiology · 2025Review
- Metabolic engineering of narrow-leafed lupin for the production of enantiomerically pure (-)-sparteine.Plant biotechnology journal · 2025Article
- Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments.Functional & integrative genomics · 2025Review
- Genome-wide identification and diversity of FAD2, FAD3 and FAE1 genes in terms of biotechnological importance in Camelina species.BMC biotechnology · 2024Article
- Integration of CRISPR/Cas9 with multi-omics technologies to engineer secondary metabolite productions in medicinal plant: Challenges and Prospects.Functional & integrative genomics · 2024Review
- Creating yellow seed Camelina sativa with enhanced oil accumulation by CRISPR-mediated disruption of Transparent Testa 8.Plant biotechnology journal · 2024Article
- Review
- CRISPR-M: Predicting sgRNA off-target effect using a multi-view deep learning network.PLoS computational biology · 2024Article
- Improving linolenic acid content in rapeseed oil by overexpression ofMolecular breeding : new strategies in plant improvement · 2024Article
- Chromosome-level assembly and analysis of Camelina neglecta: a novel diploid model for Camelina biotechnology research.Biotechnology for biofuels and bioproducts · 2024Article
- CRISPR-Cas systems and applications for crop bioengineering.Frontiers in bioengineering and biotechnology · 2024Review
67 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 nuclease system is a powerful and flexible tool for genome editing, and novel applications of this system are being developed rapidly. Here, we used CRISPR/Cas9 to target the FAD2 gene in Arabidopsis thaliana and in the closely related emerging oil seed plant, Camelina sativa, with the goal of improving seed oil composition. We successfully obtained Camelina seeds in which oleic acid content was increased from 16% to over 50% of the fatty acid composition. These increases were associated with significant decreases in the less desirable polyunsaturated fatty acids, linoleic acid (i.e. a decrease from ~16% to <4%) and linolenic acid (a decrease from ~35% to <10%). These changes result in oils that are superior on multiple levels: they are healthier, more oxidatively stable and better suited for production of certain commercial chemicals, including biofuels. As expected, A. thaliana T
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.