ArticlePloS one2016
High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 102 citations in OpenAlex.
- Towards microbiome-informed strategies for predicting and preventing pregnancy complications.Reproduction & fertility · 2026Review
- The vaginal microbiome in pregnancy and preterm birth: mechanisms, disparities, and therapeutic opportunities.NPJ biofilms and microbiomes · 2026Review
- The Role of the Vaginal Microbiome in Preterm Premature Rupture of Membranes: A Comprehensive Review of Mechanisms and Clinical Implications.Health science reports · 2025Article
- Impact of Delivery Mode on Neonatal Outcomes in Extremely Preterm Infants Born at 22 + 0 to 25 + 6 Weeks of Gestation.Medicina (Kaunas, Lithuania) · 2025Article
- Does the Vaginal Microbiota Influence the Incidence of the Preterm Premature Rupture of Membranes?Journal of clinical medicine · 2025Review
- Glutamine Attenuates Inflammation and Stimulates Amniotic Cell Proliferation in Premature Rupture of Membranes-related in vitro Models.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Changes in vaginal Ureaplasma and Lactobacillus due to antibiotic regimen for premature rupture of membranes.PloS one · 2025Article
- Interaction of vaginal microbiota and biomarkers in Premature rupture of membranes: from bench to beside.Frontiers in immunology · 2025Review
- Early prediction of preeclampsia using the first trimester vaginal microbiome.bioRxiv : the preprint server for biology · 2024Article
- Epidemiological characteristics and clinical antibiotic resistance analysis of Ureaplasma urealyticum infection among women and children in southwest China.BMC infectious diseases · 2024Article
- Microbiome in Female Reproductive Health: Implications for Fertility and Assisted Reproductive Technologies.Genomics, proteomics & bioinformatics · 2024Review
- The maternal microbiome in pregnancy, delivery, and early-stage development of neonatal microbiome after cesarean section: A prospective longitudinal study.Acta obstetricia et gynecologica Scandinavica · 2024Observational
- Review
- Assessing vaginal microbiome through Vaginal Microecology Evaluation System as a predictor forFrontiers in endocrinology · 2024Article
- Vaginal Microbial Colonization after Antibiotic Treatment in Women with Preterm Premature Rupture of Membranes: An Observational Cohort Study.Journal of clinical medicine · 2023Article
- The Female Reproductive Tract Microbiota: Friends and Foe.Life (Basel, Switzerland) · 2023Review
- Maternal Infection and Preterm Birth: From Molecular Basis to Clinical Implications.Children (Basel, Switzerland) · 2023Review
- Advances in Research on the Relationship between Vaginal Microbiota and Adverse Pregnancy Outcomes and Gynecological Diseases.Microorganisms · 2023Review
- The Gut Microbiome During Pregnancy.Maternal-fetal medicine (Wolters Kluwer Health, Inc.) · 2023Article
- Roles of the Microbiota of the Female Reproductive Tract in Gynecological and Reproductive Health.Microbiology and molecular biology reviews : MMBR · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo characterize the vaginal microbiota of women following preterm premature rupture of membranes (PPROM), and determine if microbiome composition predicts latency duration and perinatal outcomes.
designA prospective cohort study.
settingCanada. POPULATION: Women with PPROM between 24+0 and 33+6 weeks gestational age (GA).
methodsMicrobiome profiles, based on pyrosequencing of the cpn60 universal target, were generated from vaginal samples at time of presentation with PPROM, weekly thereafter, and at delivery.
main outcome measuresVaginal microbiome composition, latency duration, gestational age at delivery, perinatal outcomes.
resultsMicrobiome profiles were generated from 70 samples from 36 women. Mean GA at PPROM was 28.8 wk (mean latency 2.7 wk). Microbiome profiles were highly diverse but sequences representing Megasphaera type 1 and Prevotella spp. were detected in all vaginal samples. Only 13/70 samples were dominated by Lactobacillus spp. Microbiome profiles at the time of membrane rupture did not cluster by gestational age at PPROM, latency duration, presence of chorioamnionitis or by infant outcomes. Mycoplasma and/or Ureaplasma were detected by PCR in 81% (29/36) of women, and these women had significantly lower GA at delivery and correspondingly lower birth weight infants than Mycoplasma and/or Ureaplasma negative women.
conclusionWomen with PPROM had mixed, abnormal vaginal microbiota but the microbiome profile at PPROM did not correlate with latency duration. Prevotella spp. and Megasphaera type I were ubiquitous. The presence of Mollicutes in the vaginal microbiome was associated with lower GA at delivery. The microbiome was remarkably unstable during the latency period.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.