ArticlePloS one2016
Somite-Derived Retinoic Acid Regulates Zebrafish Hematopoietic Stem Cell Formation.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Monocyte-Derived cxcl12 Guides a Directional Migration of Blood Vessels in Zebrafish.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- Notch and retinoic acid signals regulate macrophage formation from endocardium downstream of Nkx2-5.Nature communications · 2023Article
- Zebrafish as a Model to Study Retinoic Acid Signaling in Development and Disease.Biomedicines · 2023Review
- Neuronal chemokine concentration gradients mediate effects of embryonic ethanol exposure on ectopic hypocretin/orexin neurons and behavior in zebrafish.Scientific reports · 2023Article
- Engineering a niche supporting hematopoietic stem cell development using integrated single-cell transcriptomics.Nature communications · 2022Article
- Somite morphogenesis is required for axial blood vessel formation during zebrafish embryogenesis.eLife · 2022Article
- Making Blood from the Vessel: Extrinsic and Environmental Cues Guiding the Endothelial-to-Hematopoietic Transition.Life (Basel, Switzerland) · 2021Review
- Multipotent progenitors and hematopoietic stem cells arise independently from hemogenic endothelium in the mouse embryo.Cell reports · 2021Article
- Involvement of Cxcl12a/Cxcr4b Chemokine System in Mediating the Stimulatory Effect of Embryonic Ethanol Exposure on Neuronal Density in Zebrafish Hypothalamus.Alcoholism, clinical and experimental research · 2020Article
- Location, Location, Location: How Vascular Specialization Influences Hematopoietic Fates During Development.Frontiers in cell and developmental biology · 2020Review
- Mutations in the zebrafishBlood advances · 2019Article
- Review
- Correction: Somite-Derived Retinoic Acid Regulates Zebrafish Hematopoietic Stem Cell Formation.PloS one · 2017Article
- Developmental HSC Microenvironments: Lessons from Zebrafish.Advances in experimental medicine and biology · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hematopoietic stem cells (HSCs) are multipotent progenitors that generate all vertebrate adult blood lineages. Recent analyses have highlighted the importance of somite-derived signaling factors in regulating HSC specification and emergence from dorsal aorta hemogenic endothelium. However, these factors remain largely uncharacterized. We provide evidence that the vitamin A derivative retinoic acid (RA) functions as an essential regulator of zebrafish HSC formation. Temporal analyses indicate that RA is required for HSC gene expression prior to dorsal aorta formation, at a time when the predominant RA synthesis enzyme, aldh1a2, is strongly expressed within the paraxial mesoderm and somites. Previous research implicated the Cxcl12 chemokine and Notch signaling pathways in HSC formation. Consequently, to understand how RA regulates HSC gene expression, we surveyed the expression of components of these pathways in RA-depleted zebrafish embryos. During somitogenesis, RA-depleted embryos exhibit altered expression of jam1a and jam2a, which potentiate Notch signaling within nascent endothelial cells. RA-depleted embryos also exhibit a severe reduction in the expression of cxcr4a, the predominant Cxcl12b receptor. Furthermore, pharmacological inhibitors of RA synthesis and Cxcr4 signaling act in concert to reduce HSC formation. Our analyses demonstrate that somite-derived RA functions to regulate components of the Notch and Cxcl12 chemokine signaling pathways during HSC formation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.