Evidence map›Paper›PMID 27858580›Full record

ArticleRNA biology2017

Toxins MazF and MqsR cleave Escherichia coli rRNA precursors at multiple sites.

Toomas Mets, Markus Lippus, David Schryer, Aivar Liiv, Villu Kasari, Anton Paier, Ülo Maiväli, Jaanus Remme, Tanel Tenson, Niilo Kaldalu

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 47 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. Are all antibiotic persisters created equal?Frontiers in cellular and infection microbiology · 2022
    Review
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Toomas Metsa Institute of Technology, University of Tartu , Tartu , Estonia.
Markus Lippusa Institute of Technology, University of Tartu , Tartu , Estonia.
David Schryera Institute of Technology, University of Tartu , Tartu , Estonia.
Aivar Liivb Institute of Molecular and Cell Biology, University of Tartu , Tartu , Estonia.
Villu Kasaria Institute of Technology, University of Tartu , Tartu , Estonia.
Anton Paiera Institute of Technology, University of Tartu , Tartu , Estonia.
Ülo Maivälia Institute of Technology, University of Tartu , Tartu , Estonia.
Jaanus Remmeb Institute of Molecular and Cell Biology, University of Tartu , Tartu , Estonia.
Tanel Tensona Institute of Technology, University of Tartu , Tartu , Estonia.
Niilo Kaldalua Institute of Technology, University of Tartu , Tartu , Estonia.
University of Tartu · EE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The endoribonuclease toxins of the E. coli toxin-antitoxin systems arrest bacterial growth and protein synthesis by targeting cellular mRNAs. As an exception, E. coli MazF was reported to cleave also 16S rRNA at a single site and separate an anti-Shine-Dalgarno sequence-containing RNA fragment from the ribosome. We noticed extensive rRNA fragmentation in response to induction of the toxins MazF and MqsR, which suggested that these toxins can cleave rRNA at multiple sites. We adapted differential RNA-sequencing to map the toxin-cleaved 5'- and 3'-ends. Our results show that the MazF and MqsR cleavage sites are located within structured rRNA regions and, therefore, are not accessible in assembled ribosomes. Most of the rRNA fragments are located in the aberrant ribosomal subunits that accumulate in response to toxin induction and contain unprocessed rRNA precursors. We did not detect MazF- or MqsR-cleaved rRNA in stationary phase bacteria and in assembled ribosomes. Thus, we conclude that MazF and MqsR cleave rRNA precursors before the ribosomes are assembled and potentially facilitate the decay of surplus rRNA transcripts during stress.

Indexed as

Bacterial ToxinsBinding SitesDNA-Binding ProteinsEndoribonucleasesEscherichia coliEscherichia coli ProteinsModels, MolecularNucleic Acid ConformationPhosphorylationProtein BindingProtein ConformationRNA, BacterialRNA CleavageRNA PrecursorsRNA, RibosomalSequence Analysis, RNABacterial ToxinsDNA-Binding ProteinsEndoribonucleasesEscherichia coli ProteinsMazF protein, E coliMqsR protein, E coliRNA, BacterialRNA PrecursorsRNA, RibosomalDifferential RNA sequencingMazFMqsRribosomerRNA precursorsToxin-Antitoxin systems

Identifiers

PMID27858580
PMCPMC5270532
OpenAlexW2552311778

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.