ArticleEpigenetics2017
Tracking the evolution of epialleles during neural differentiation and brain development: D-Aspartate oxidase as a model gene.
Article in Epigenetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- DNA methylation patterns re-establish during clonal expansion restoring cell-to-cell epialleles heterogeneity.Biology direct · 2026Article
- A specific pluripotency-associated eRNA controls Nanog locus by shaping the epigenetic landscape and stabilizing enhancer-promoter interaction.Nucleic acids research · 2025Article
- Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation.International journal of molecular sciences · 2023Article
- MC profiling: a novel approach to analyze DNA methylation heterogeneity in genome-wide bisulfite sequencing data.NAR genomics and bioinformatics · 2022Article
- Epialleles and epiallelic heterogeneity in hematological malignancies.Medical oncology (Northwood, London, England) · 2022Review
- Epigenetic remodelling of Fxyd1 promoters in developing heart and brain tissues.Scientific reports · 2022Article
- DNA methylation impact on Fabry disease.Clinical epigenetics · 2021Review
- Generation of High-Yield, Functional Oligodendrocytes from a c-International journal of molecular sciences · 2021Article
- DNA Methylation Profiles ofBiomolecules · 2021Article
- Human D-aspartate Oxidase: A Key Player in D-aspartate Metabolism.Frontiers in molecular biosciences · 2021Article
- TheToxins · 2020Article
- New Evidence on the Role of D-Aspartate Metabolism in Regulating Brain and Endocrine System Physiology: From Preclinical Observations to Clinical Applications.International journal of molecular sciences · 2020Review
- Modeling DNA Methylation Profiles through a Dynamic Equilibrium between Methylation and Demethylation.Biomolecules · 2020Article
- Article
- Selective demethylation of two CpG sites causes postnatal activation of the Dao gene and consequent removal of D-serine within the mouse cerebellum.Clinical epigenetics · 2019Article
- TheCancers · 2019Article
- Allele-specific epigenome maps reveal sequence-dependent stochastic switching at regulatory loci.Science (New York, N.Y.) · 2018Article
- DNA methylation landscape of the genes regulating D-serine and D-aspartate metabolism in post-mortem brain from controls and subjects with schizophrenia.Scientific reports · 2018Article
- Combined neurodevelopmental exposure to deltamethrin and corticosterone is associated with Nr3c1 hypermethylation in the midbrain of male mice.Neurotoxicology and teratologyArticle
Corrections and comments
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Authors and funding
20 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We performed ultra-deep methylation analysis at single molecule level of the promoter region of developmentally regulated D-Aspartate oxidase (Ddo), as a model gene, during brain development and embryonic stem cell neural differentiation. Single molecule methylation analysis enabled us to establish the effective epiallele composition within mixed or pure brain cell populations. In this framework, an epiallele is defined as a specific combination of methylated CpG within Ddo locus and can represent the epigenetic haplotype revealing a cell-to-cell methylation heterogeneity. Using this approach, we found a high degree of polymorphism of methylated alleles (epipolymorphism) evolving in a remarkably conserved fashion during brain development. The different sets of epialleles mark stage, brain areas, and cell type and unravel the possible role of specific CpGs in favoring or inhibiting local methylation. Undifferentiated embryonic stem cells showed non-organized distribution of epialleles that apparently originated by stochastic methylation events on individual CpGs. Upon neural differentiation, despite detecting no changes in average methylation, we observed that the epiallele distribution was profoundly different, gradually shifting toward organized patterns specific to the glial or neuronal cell types. Our findings provide a deep view of gene methylation heterogeneity in brain cell populations promising to furnish innovative ways to unravel mechanisms underlying methylation patterns generation and alteration in brain diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.