ArticleEpigenetics2017
High fat diet and exercise lead to a disrupted and pathogenic DNA methylome in mouse liver.
Article in Epigenetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- The Influence of Epigenetic Modifications on Metabolic Changes in White Adipose Tissue and Liver and Their Potential Impact in Exercise.Frontiers in physiology · 2021Pooled it
- Crosstalk BetweenCancers · 2026Review
- Higher mitochondrial DNA methylation is associated with increased risk of stroke: a nested case-control study.Human genetics · 2026Article
- Diet intervention altered DNA methylation of potassium voltage-gated channel subfamily Q member 1 in metabolic dysfunction-associated steatohepatitis.Clinical epigenetics · 2026Article
- Protective effect of exercise on metabolic dysfunction‑associated fatty liver disease: Potential epigenetic mechanisms (Review).International journal of molecular medicine · 2025Review
- Evaluation for CpG island methylation of FASN and SREBP promoter in Mongolian gerbil NAFLD model.Archives of medical science : AMS · 2025Article
- Paternally multi-generational high-fat diet causes obesity and metabolic disorder through intergenerational DNA methylation.Frontiers in nutrition · 2025Article
- Bibliometric analysis of research on the utilization of nanotechnology in diabetes mellitus and its complications.Nanomedicine (London, England) · 2024Article
- Epigenetic modifications in obesity-associated diseases.MedComm · 2024Review
- Sweating it out: How physical activity can combat high genetic risk for nonalcoholic fatty liver disease.Liver international : official journal of the International Association for the Study of the Liver · 2023Article
- [Factors influencing the clinical phenotype of hepatolenticular degeneration].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2023Review
- Short-term in vivo testing to discriminate genotoxic carcinogens from non-genotoxic carcinogens and non-carcinogens using next-generation RNA sequencing, DNA microarray, and qPCR.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2023Review
- Restoring Epigenetic Reprogramming with Diet and Exercise to Improve Health-Related Metabolic Diseases.Biomolecules · 2023Review
- Review
- Impact of Physical Activity and Exercise on the Epigenome in Skeletal Muscle and Effects on Systemic Metabolism.Biomedicines · 2022Review
- Proteomic analysis of the effect of high-fat-diet and voluntary physical activity on mouse liver.PloS one · 2022Article
- Cafeteria diet induces global andAdipocyte · 2021Article
- Effect of exercise and diet intervention in NAFLD and NASH via GAB2 methylation.Cell & bioscience · 2021Article
- Environmental and socio-cultural impacts on global DNA methylation in the indigenous Huichol population of Nayarit, Mexico.Environmental science and pollution research international · 2021Article
- NAFLD and Physical Exercise: Ready, Steady, Go!Frontiers in nutrition · 2021Review
Corrections and comments
- Erratum issuedErratum.2017
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
High-fat diet consumption and sedentary lifestyle elevates risk for obesity, non-alcoholic fatty liver disease, and cancer. Exercise training conveys health benefits in populations with or without these chronic conditions. Diet and exercise regulate gene expression by mediating epigenetic mechanisms in many tissues; however, such effects are poorly documented in the liver, a central metabolic organ. To dissect the consequences of diet and exercise on the liver epigenome, we measured DNA methylation, using reduced representation bisulfite sequencing, and transcription, using RNA-seq, in mice maintained on a fast food diet with sedentary lifestyle or exercise, compared with control diet with and without exercise. Our analyses reveal that genome-wide differential DNA methylation and expression of gene clusters are induced by diet and/or exercise. A combination of fast food and exercise triggers extensive gene alterations, with enrichment of carbohydrate/lipid metabolic pathways and muscle developmental processes. Through evaluation of putative protective effects of exercise on diet-induced DNA methylation, we show that hypermethylation is effectively prevented, especially at promoters and enhancers, whereas hypomethylation is only partially attenuated. We assessed diet-induced DNA methylation changes associated with liver cancer-related epigenetic modifications and identified significant increases at liver-specific enhancers in fast food groups, suggesting partial loss of liver cell identity. Hypermethylation at a subset of gene promoters was associated with inhibition of tissue development and promotion of carcinogenic processes. Our study demonstrates extensive reprogramming of the epigenome by diet and exercise, emphasizing the functional relevance of epigenetic mechanisms as an interface between lifestyle modifications and phenotypic alterations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.