ReviewWorld journal of microbiology & biotechnology2017
Recent advances and challenges in the heterologous production of microbial nitrilases for biocatalytic applications.
Review in World journal of microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 59 citations in OpenAlex.
- Novel Haloalkaliphilic Nitrile-Degrading Bacteria From Soda Lake Soil of Rift Valley Kenya.TheScientificWorldJournal · 2025Article
- Nitrilase GiNIT fromInternational journal of molecular sciences · 2024Article
- Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity.Chemical reviews · 2023Review
- Biodegradation of nitriles derived from glucosinolates in rapeseed meal by BnNIT2: a nitrilase from Brassica napus with wide substrate specificity.Applied microbiology and biotechnology · 2022Article
- Metabolism of Aldoximes and Nitriles in Plant-Associated Bacteria and Its Potential in Plant-Bacteria Interactions.Microorganisms · 2022Review
- Recent Advances in Enzymes for the Bioremediation of Pollutants.Biochemistry research international · 2021Review
- Plant Nitrilase Homologues in Fungi: Phylogenetic and Functional Analysis with Focus on Nitrilases inMolecules (Basel, Switzerland) · 2020Article
- Genetic and Functional Diversity of Nitrilases in Agaricomycotina.International journal of molecular sciences · 2019Article
- Comparative Analysis of the Conversion of Mandelonitrile and 2-Phenylpropionitrile by a Large Set of Variants Generated from a Nitrilase Originating fromMolecules (Basel, Switzerland) · 2019Review
- A review on remediation of cyanide containing industrial wastes using biological systems with special reference to enzymatic degradation.World journal of microbiology & biotechnology · 2019Review
- The use of clade-specific PCR assays to identify novel nitrilase genes from environmental isolates.MicrobiologyOpen · 2019Article
- Cryo-EM and directed evolution reveal howCommunications biology · 2019Article
- Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942.Applied and environmental microbiology · 2018Article
- Cloning, overexpression, and characterization of a thermostable nitrilase from an Antarctic Pyrococcus sp.Extremophiles : life under extreme conditions · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study is to review the current state of and highlight the challenges in the production of microbial nitrilases as catalysts for the mild hydrolysis of industrially important nitriles. Together with aldoxime dehydratase, the nitrile-hydrolyzing enzymes (nitrilase, nitrile hydratase) are key enzymes in the aldoxime-nitrile pathway which is widely distributed in bacteria and fungi. The availability of nitrilases has grown significantly over the past decade due to the use of metagenomic and database-mining approaches. Databases contain plenty of putative enzymes of this type, whose overproduction may improve the spectrum and the industrial utility of nitrilases. By exploiting this resource, the number of experimentally verified nitrilases has recently increased to several hundred. We especially focus on the efficient heterologous expression systems that are applicable for the overproduction of wild-type nitrilases and their artificial variants. Biocatalyst forms with industrial potential are also highlighted. The potential industrial applications of nitrilases are classified according to their target products (α-hydroxy acids, α- and β-amino acids, cyano acids, amides). The emerging uses of nitrilases and their subtypes (cyanide hydratases, cyanide dihydratases) in bioremediation is also summarized. The integration of nitrilases with other enzymes into artificial multienzymatic and chemoenzymatic pathways is considered a promising strategy for future applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.