ArticleFrontiers in microbiology2016
Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Microbiota-targeted interventions and clinical implications for maternal-offspring health: An umbrella review of systematic reviews and meta-analyses of randomised controlled trials.Journal of global health · 2024Pooled it
- Identification ofFoods (Basel, Switzerland) · 2026Article
- Occurrence and applications of CRISPR-Cas systems in bifidobacteria.Applied and environmental microbiology · 2026Review
- Safety and efficacy concerns in Indian probiotics: Insights from whole-genome sequencing andGut microbes reports · 2026Article
- Storage temperature as a determinant of probiotic long-term stability in commercial dietary supplements.Frontiers in nutrition · 2026Article
- Article
- The curated Lactobacillus acidophilus NCFM genome provides insights into strain specificity and microevolution.BMC genomics · 2025Article
- The Safety of Probiotics Intended for Use in Pregnant and Lactating Women: From a Desirable to a Required Task.Foods (Basel, Switzerland) · 2024Review
- Nanobodies as potential tools for microbiological testing of live biotherapeutic products.AMB Express · 2024Article
- A systematic scoping review of study methodology for randomized controlled trials investigating probiotics in athletic and physically active populations.Journal of sport and health science · 2024Article
- Real-time PCR methods for identification and stability monitoring ofFrontiers in microbiology · 2024Article
- Rapid identification of lactic acid bacteria at species/subspecies level via ensemble learning of Ramanomes.Frontiers in microbiology · 2024Article
- Insights from metagenome-assembled genomes on the genetic stability and safety of over-the-counter probiotic products.Current genetics · 2023Article
- Article
- Labeling compliance and online claims for Ayurvedic herbal supplements on the U.S. market associated with the purported treatment of COVID-19.Food control · 2023Article
- The power of DNA based methods in probiotic authentication.Frontiers in microbiology · 2023Article
- Infection prevention for extremely low birth weight infants in the NICU.Seminars in fetal & neonatal medicine · 2022Review
- Compositional Quality and Possible Gastrointestinal Performance of Marketed Probiotic Supplements.Probiotics and antimicrobial proteins · 2022Article
- Real-time polymerase chain reaction methods for strain specific identification and enumeration of strainFrontiers in microbiology · 2022Article
- Enumeration of Probiotic Strain Lacticaseibacillus rhamnosus GG (ATCC 53103) Using Viability Real-time PCR.Probiotics and antimicrobial proteins · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in microbiome research have brought renewed focus on beneficial bacteria, many of which are available in food and dietary supplements. Although probiotics have historically been defined as microorganisms that convey health benefits when ingested in sufficient viable amounts, this description now includes the stipulation "well defined strains," encompassing definitive taxonomy for consumer consideration and regulatory oversight. Here, we evaluated 52 commercial dietary supplements covering a range of labeled species using plate counting and targeted genotyping. Strain identities were assessed using methods recently published by the United States Pharmacopeial Convention. We also determined the relative abundance of individual bacteria by high-throughput sequencing (HTS) of the 16S rRNA sequence using paired-end 2 × 250 bp Illumina MiSeq technology. Using these methods, we tested the hypothesis that products do contain the quantitative and qualitative list of labeled microbial species. We found that 17 samples (33%) were below label claim for CFU prior to their expiration dates. A multiplexed-PCR scheme showed that only 30/52 (58%) of the products contained a correctly labeled classification, with issues encompassing incorrect taxonomy, missing species, and un-labeled species. The HTS revealed that many blended products consisted predominantly of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.