Evidence map›Paper›PMID 27857576›Full record

ArticleBiophysics (Nagoya-shi, Japan)2009

Stochastic emergence of multiple intermediates detected by single-molecule quasi-static mechanical unfolding of protein.

Akihiro Fukagawa, Michio Hiroshima, Isao Sakane, Makio Tokunaga

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysics (Nagoya-shi, Japan), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Akihiro FukagawaStructural Biology Center, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan; Research Center for Allergy and Immunology, RIKEN, Yokohama, Kanagawa 230-0045, Japan.
Michio HiroshimaStructural Biology Center, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan; Research Center for Allergy and Immunology, RIKEN, Yokohama, Kanagawa 230-0045, Japan.
Isao SakaneStructural Biology Center, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.
Makio TokunagaStructural Biology Center, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan; Research Center for Allergy and Immunology, RIKEN, Yokohama, Kanagawa 230-0045, Japan; Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (Sokendai), Mishima, Shizuoka 411-8540, Japan; Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori, Yokohama, Kanagawa 226-8501, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Experimental probing of a protein-folding energy landscape can be challenging, and energy landscapes comprising multiple intermediates have not yet been defined. Here, we quasi-statically unfolded single molecules of staphylococcal nuclease by constant-rate mechanical stretching with a feedback positioning system. Multiple discrete transition states were detected as force peaks, and only some of the multiple transition states emerged stochastically in each trial. This finding was confirmed by molecular dynamics simulations, and agreed with another result of the simulations which showed that individual trajectories took highly heterogeneous pathways. The presence of Ca

Indexed as

energy landscapemolecular dynamicsmolecular force microscopyprotein foldingsingle-molecule detection

Identifiers

PMID27857576
PMCPMC5036639

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.