ArticleScientific reports2016
Synthesis of (3R)-acetoin and 2,3-butanediol isomers by metabolically engineered Lactococcus lactis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- The Effectiveness of Brief Meditation Assisted with Cardiac Biofeedback on Interoceptive Accuracy, Sensibility and Awareness: A Randomized Controlled Trial.Applied psychophysiology and biofeedback · 2026Trial
- Process scale-up simulation and techno-economic assessment of ethanol fermentation from cheese whey.Biotechnology for biofuels and bioproducts · 2024Article
- Application of microbial resources in biorefineries: Current trend and future prospects.Heliyon · 2024Review
- A Point Mutation in Cassette Relieves the Repression Regulation of CcpA Resulting in an Increase in the Degradation of 2,3-Butanediol inMicroorganisms · 2024Article
- Evaluation of 2,3-butanediol derived from whey fermentation as an effective bio-based monomer for waterborne polyurethane dispersions.Frontiers in chemistry · 2024Article
- Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase.Microbial cell factories · 2023Review
- Article
- Metabolic Engineering ofMicroorganisms · 2023Article
- Metabolic engineering for the production of acetoin and 2,3-butanediol at elevated temperature inFrontiers in bioengineering and biotechnology · 2023Article
- Regulatory role of cysteines in (2R, 3R)-butanediol dehydrogenase BdhA of Bacillus velezensis strain GH1-13.Journal of microbiology (Seoul, Korea) · 2022Article
- Article
- Mechanisms of Acetoin Toxicity and Adaptive Responses in an Acetoin-Producing Species, Lactococcus lactis.Applied and environmental microbiology · 2021Article
- Metabolic engineering of non-pathogenic microorganisms for 2,3-butanediol production.Applied microbiology and biotechnology · 2021Review
- C4 Bacterial Volatiles Improve Plant Health.Pathogens (Basel, Switzerland) · 2021Review
- Increasing ATP turnover boosts productivity of 2,3-butanediol synthesis in Escherichia coli.Microbial cell factories · 2021Article
- Phylogenetics-based identification and characterization of a superior 2,3-butanediol dehydrogenase for Zymomonas mobilis expression.Biotechnology for biofuels · 2020Article
- Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca.Microbial cell factories · 2020Article
- Respiratory Physiology of Lactococcus lactis in Chemostat Cultures and Its Effect on Cellular Robustness in Frozen and Freeze-Dried Starter Cultures.Applied and environmental microbiology · 2020Article
- Harnessing biocompatible chemistry for developing improved and novel microbial cell factories.Microbial biotechnology · 2020Review
- Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol.Biotechnology for biofuels · 2020Article
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Authors and funding
5 authors.
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Abstract
The potential that lies in harnessing the chemical synthesis capabilities inherent in living organisms is immense. Here we demonstrate how the biosynthetic machinery of Lactococcus lactis, can be diverted to make (3R)-acetoin and the derived 2,3-butanediol isomers meso-(2,3)-butanediol (m-BDO) and (2R,3R)-butanediol (R-BDO). Efficient production of (3R)-acetoin was accomplished using a strain where the competing lactate, acetate and ethanol forming pathways had been blocked. By introducing different alcohol dehydrogenases into this strain, either EcBDH from Enterobacter cloacae or SadB from Achromobacter xylosooxidans, it was possible to achieve high-yield production of m-BDO or R-BDO respectively. To achieve biosustainable production of these chemicals from dairy waste, we transformed the above strains with the lactose plasmid pLP712. This enabled efficient production of (3R)-acetoin, m-BDO and R-BDO from processed whey waste, with titers of 27, 51, and 32 g/L respectively. The corresponding yields obtained were 0.42, 0.47 and 0.40 g/g lactose, which is 82%, 89%, and 76% of maximum theoretical yield respectively. These results clearly demonstrate that L. lactis is an excellent choice as a cell factory for transforming lactose containing dairy waste into value added chemicals.
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