ArticleScientific reports2016
Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 169 citations in OpenAlex.
- Utilization of phosphate-responsive promoters for auto-inducible management of biotechnological processes based on cyanobacteria.Metabolic engineering communications · 2026Article
- Rhodo-Box: A Synthetic Biology Toolbox to Facilitate Metabolic Engineering ofACS synthetic biology · 2026Article
- Deciphering the metabolic details of L-lysine toxicity in cyanobacteria.Plant physiology · 2026Article
- Establishing a Malonyl-CoA Biosensor for the Two Model CyanobacteriaACS synthetic biology · 2025Article
- qByte: An open-source isothermal fluorimeter for democratizing analysis of nucleic acids, proteins and cells.PLoS biology · 2025Article
- Co-expression of auxiliary genes enhances the activity of a heterologous OBiotechnology for biofuels and bioproducts · 2025Article
- Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics: A comprehensive review.Environmental science and ecotechnology · 2024Review
- Phycobilisome protein ApcG interacts with PSII and regulates energy transfer in Synechocystis.Plant physiology · 2024Article
- Rerouting phytosterol degradation pathway for directed androst-1,4-diene-3,17-dione microbial bioconversion.Applied microbiology and biotechnology · 2024Article
- Fine-Tuning Gene Expression in Bacteria by Synthetic Promoters.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803.Biotechnology for biofuels and bioproducts · 2023Article
- Design of synthetic promoters for cyanobacteria with generative deep-learning model.Nucleic acids research · 2023Article
- Promoting Heme and Phycocyanin Biosynthesis inMarine drugs · 2023Article
- Interlaboratory Reproducibility in Growth and Reporter Expression in the CyanobacteriumACS synthetic biology · 2023Article
- Identification of a Far-Red Light-Inducible Promoter that Exhibits Light Intensity Dependency and Reversibility in a Cyanobacterium.ACS synthetic biology · 2023Article
- Mapping competitive pathways to terpenoid biosynthesis in Synechocystis sp. PCC 6803 using an antisense RNA synthetic tool.Microbial cell factories · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Toward a synthetic hydrogen sensor in cyanobacteria: Functional production of an oxygen-tolerant regulatory hydrogenase inFrontiers in microbiology · 2023Article
- Expression and activity of heterologous hydroxyisocaproate dehydrogenases inEngineering microbiology · 2022Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For effective metabolic engineering, a toolbox of genetic components that enables predictable control of gene expression is needed. Here we present a systematic study of promoters and ribosome binding sites in the unicellular cyanobacterium Synechocystis sp. PCC 6803. A set of metal ion inducible promoters from Synechocystis were compared to commonly used constitutive promoters, by measuring fluorescence of a reporter protein in a standardized setting to allow for accurate comparisons of promoter activity. The most versatile and useful promoter was found to be PnrsB, which from a relatively silent expression could be induced almost 40-fold, nearly up to the activity of the strong psbA2 promoter. By varying the concentrations of the two metal ion inducers Ni
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