ArticlePlant physiology2017
Homologues of the RPW8 Resistance Protein Are Localized to the Extrahaustorial Membrane that Is Likely Synthesized De Novo.
Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- The role of MLO in powdery mildew susceptibility depends on a combination of functional specialization and subcellular localization.Plant physiology · 2026Article
- Genome-Wide Identification Analysis of theGenes · 2025Article
- A powdery mildew core effector protein targets the host endosome tethering complexes HOPS and CORVET in barley.Plant physiology · 2025Article
- Interplay of transport vesicles during plant-fungal pathogen interaction.Stress biology · 2023Review
- Autophagy and multivesicular body pathways cooperate to protect sulfur assimilation and chloroplast functions.Plant physiology · 2023Article
- The endosome as an effector target to mediate plant immunity?Journal of experimental botany · 2023Article
- The haustorium: The root of biotrophic fungal pathogens.Frontiers in plant science · 2022Review
- Genetic Architecture of Powdery Mildew Resistance Revealed by a Genome-Wide Association Study of a Worldwide Collection of Flax (Frontiers in plant science · 2022Article
- Computational models for prediction of protein-protein interaction in rice andFrontiers in plant science · 2022Article
- Regulation and Function of Defense-Related Callose Deposition in Plants.International journal of molecular sciences · 2021Review
- Advancement of research on plant NLRs evolution, biochemical activity, structural association, and engineering.Planta · 2020Review
- Focal Accumulation of ROS Can BlockInternational journal of molecular sciences · 2020Article
- Specific Recruitment of Phosphoinositide Species to the Plant-Pathogen Interfacial Membrane Underlies Arabidopsis Susceptibility to Fungal Infection.The Plant cell · 2020Article
- Blast resistance gene Pi54 over-expressed in rice to understand its cellular and sub-cellular localization and response to different pathogens.Scientific reports · 2020Article
- Coordination and Crosstalk between Autophagosome and Multivesicular Body Pathways in Plant Stress Responses.Cells · 2020Review
- A Diverse Membrane Interaction Network for Plant Multivesicular Bodies: Roles in Proteins Vacuolar Delivery and Unconventional Secretion.Frontiers in plant science · 2020Review
- Phaeophyceaean (Brown Algal) Extracts Activate Plant Defense Systems inFrontiers in plant science · 2020Article
- RPW8/HR repeats control NLR activation in Arabidopsis thaliana.PLoS genetics · 2019Article
- Modulation of ACD6 dependent hyperimmunity by natural alleles of an Arabidopsis thaliana NLR resistance gene.PLoS genetics · 2018Article
- Arabidopsis phospholipase Dα1 and Dδ oppositely modulate EDS1- and SA-independent basal resistance against adapted powdery mildew.Journal of experimental botany · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Upon penetration of the host cell wall, the powdery mildew fungus develops a feeding structure named the haustorium in the invaded host cell. Concomitant with haustorial biogenesis, the extrahaustorial membrane (EHM) is formed to separate the haustorium from the host cell cytoplasm. The Arabidopsis resistance protein RPW8.2 is specifically targeted to the EHM where it activates haustorium-targeted resistance against powdery mildew. RPW8.2 belongs to a small family with six members in Arabidopsis (Arabidopsis thaliana). Whether Homologs of RPW8 (HR) 1 to HR4 are also localized to the EHM and contribute to resistance has not been determined. Here, we report that overexpression of HR1, HR2, or HR3 led to enhanced resistance to powdery mildew, while genetic depletion of HR2 or HR3 resulted in enhanced susceptibility, indicating that these RPW8 homologs contribute to basal resistance. Interestingly, we found that N-terminally YFP-tagged HR1 to HR3 are also EHM-localized. This suggests that EHM-targeting is an ancestral feature of the RPW8 family. Indeed, two RPW8 homologs from Brassica oleracea tested also exhibit EHM-localization. Domain swapping analysis between HR3 and RPW8.2 suggests that sequence diversification in the N-terminal 146 amino acids of RPW8.2 probably functionally distinguishes it from other family members. Moreover, we found that N-terminally YFP-tagged HR3 is also localized to the plasma membrane and the fungal penetration site (the papilla) in addition to the EHM. Using this unique feature of YFP-HR3, we obtained preliminary evidence to suggest that the EHM is unlikely derived from invagination of the plasma membrane, rather it may be mainly synthesized de novo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.