ArticleGenome research2017
The dynamic landscape of fission yeast meiosis alternative-splice isoforms.
Article in Genome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Structural duality enables a single protein to act as a toxin-antidote pair for meiotic drive.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Evolutionary Modes of wtf Meiotic Driver Genes in Schizosaccharomyces pombe.Genome biology and evolution · 2024Article
- A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast.PLoS genetics · 2024Article
- E3 SUMO ligase SIZ1 splicing variants localize and function according to external conditions.Plant physiology · 2024Article
- The fission yeast methyl phosphate capping enzyme Bmc1 guides 2'-O-methylation of the U6 snRNA.Nucleic acids research · 2023Article
- A microbiota-epigenetic circuit controls systematic circadian programs in the gut epithelium.Frontiers in systems biology · 2023Article
- S. pombe wtf drivers use dual transcriptional regulation and selective protein exclusion from spores to cause meiotic drive.PLoS genetics · 2022Article
- Emerging Function of Ecotype-Specific Splicing in the Recruitment of Commensal Microbiome.International journal of molecular sciences · 2022Review
- Alternative splicing diversifies the transcriptome and proteome of the rice blast fungus during host infection.RNA biology · 2022Article
- Article
- PlantSPEAD: a web resource towards comparatively analysing stress-responsive expression of splicing-related proteins in plant.Plant biotechnology journal · 2021Article
- TheeLife · 2020Article
- SMRT sequencing of the full-length transcriptome of Odontotermes formosanus (Shiraki) under Serratia marcescens treatment.Scientific reports · 2020Article
- Improving the diversity of captured full-length isoforms using a normalized single-molecule RNA-sequencing method.Communications biology · 2020Article
- Full-Length Transcript-Based Proteogenomics of Rice Improves Its Genome and Proteome Annotation.Plant physiology · 2020Article
- Dramatically diverse Schizosaccharomyces pombe wtf meiotic drivers all display high gamete-killing efficiency.PLoS genetics · 2020Article
- Barley Anther and Meiocyte Transcriptome Dynamics in Meiotic Prophase I.Frontiers in plant science · 2020Article
- The changing paradigm of intron retention: regulation, ramifications and recipes.Nucleic acids research · 2019Review
- Killer Meiotic Drive and Dynamic Evolution of the wtf Gene Family.Molecular biology and evolution · 2019Article
- Hybrid sequencing-based personal full-length transcriptomic analysis implicates proteostatic stress in metastatic ovarian cancer.Oncogene · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative splicing increases the diversity of transcriptomes and proteomes in metazoans. The extent to which alternative splicing is active and functional in unicellular organisms is less understood. Here, we exploit a single-molecule long-read sequencing technique and develop an open-source software program called SpliceHunter to characterize the transcriptome in the meiosis of fission yeast. We reveal 14,353 alternative splicing events in 17,669 novel isoforms at different stages of meiosis, including antisense and read-through transcripts. Intron retention is the major type of alternative splicing, followed by alternate "intron in exon." Seven hundred seventy novel transcription units are detected; 53 of the predicted proteins show homology in other species and form theoretical stable structures. We report the complexity of alternative splicing along isoforms, including 683 intra-molecularly co-associated intron pairs. We compare the dynamics of novel isoforms based on the number of supporting full-length reads with those of annotated isoforms and explore the translational capacity and quality of novel isoforms. The evaluation of these factors indicates that the majority of novel isoforms are unlikely to be both condition-specific and translatable but consistent with the possibility of biologically functional novel isoforms. Moreover, the co-option of these unusual transcripts into newly born genes seems likely. Together, the results of this study highlight the diversity and dynamics at the isoform level in the sexual development of fission yeast.
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