Evidence map›Paper›PMID 27856278›Full record

ArticleJournal of microbiological methods2017

Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in <25min.

Farhan Ahmad, Robert D Stedtfeld, Hassan Waseem, Maggie R Williams, Alison M Cupples, James M Tiedje, Syed A Hashsham

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiological methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
  4. Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022
    Review
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Farhan AhmadDepartment of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
Robert D StedtfeldDepartment of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
Hassan WaseemDepartment of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
Maggie R WilliamsDepartment of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
Alison M CupplesDepartment of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
James M TiedjeThe Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
Syed A HashshamDepartment of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA; The Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA. Electronic address: hashsham@egr.msu.edu.

Funding

VOLATILE ORGANIC CONTAMINANTS--QUANTIFYING THEIR MOVEMENT IN THE UNSATURATED ZONEP42ES004911 · NIEHS · MICHIGAN STATE UNIVERSITY · PI Timothy R. Zacharewski · 1989 to 2026
$64.9M
NIEHS NIH HHS P42 ES004911
6 · The paper itself

Abstract

We are reporting a most probable number approach integrated to loop mediated isothermal technique (MPN-LAMP) focusing on Gram-negative Escherichia coli and Gram-positive Enterococcus faecalis bacterial cells without nucleic acids extraction. LAMP assays for uidA from E. coli and gelE from E. faecalis were successfully performed directly on cells up to single digit concentration using a commercial real time PCR instrument. Threshold time values of LAMP assays of bacterial cells, heat treated bacterial cells (95°C for 5min), and their purified genomic DNA templates were similar, implying that amplification could be achieved directly from bacterial cells at 63°C. Viability of bacterial cells was confirmed by using propidium monoazide in a LAMP assay with E. faecalis. To check its functionality on a microfluidic platform, MPN-LAMP assays targeting <10CFU of bacteria were also translated onto polymeric microchips and monitored by a low-cost fluorescence imaging system. The overall system provided signal-to-noise (SNR) ratios up to 800, analytical sensitivity of <10CFU, and time to positivity of about 20min. MPN-LAMP assays were performed for cell concentrations in the range of 10

Indexed as

Water MicrobiologyAzidesColony Count, MicrobialDNA, BacterialEnterococcus faecalisEscherichia coliFood ContaminationFood MicrobiologyGenes, BacterialMicrobial ViabilityMicrofluidic Analytical TechniquesPropidiumReal-Time Polymerase Chain ReactionSensitivity and SpecificitySignal-To-Noise RatioAzidesDNA, BacterialPropidiumpropidium monoazideDetection limitLoop-mediated isothermal amplificationMost probable numberPoint-of-care diagnosticsPropidium monoazide

Identifiers

PMID27856278
PMCPMC5195917

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.