Evidence map›Paper›PMID 27855189›Full record

ArticlePloS one2016

Genomic and Transcriptomic Alterations Associated with STAT3 Activation in Head and Neck Cancer.

Noah D Peyser, Kelsey Pendleton, William E Gooding, Vivian W Y Lui, Daniel E Johnson, Jennifer R Grandis

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Modulators of Redox Metabolism in Head and Neck Cancer.Antioxidants & redox signaling · 2018
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Noah D PeyserDepartment of Otolaryngology-Head and Neck Surgery, University of California San Francisco, San Francisco, CA, United States of America, 94143.
Kelsey PendletonDepartment of Otolaryngology, University of Pittsburgh and the University of Pittsburgh Cancer Institute, Pittsburgh, PA, United States of America, 15213.
William E GoodingBiostatistics Facility, University of Pittsburgh Cancer Institute, Pittsburgh, PA, United States of America, 15213.
Vivian W Y LuiDepartment of Pharmacology and Pharmacy, School of Biomedical Sciences, Li-Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong SAR, China.
Daniel E JohnsonDepartment of Medicine, University of Pittsburgh and the University of Pittsburgh Cancer Institute, Pittsburgh, PA, United States of America.
Jennifer R GrandisDepartment of Otolaryngology-Head and Neck Surgery, University of California San Francisco, San Francisco, CA, United States of America, 94143.
UPMC Hillman Cancer Center · USUniversity of California, San Francisco · USUniversity of Hong Kong · HK

Funding

WILD TYPE P53-BASED ADJUVANT IMMUNOTHERAPY FOR SCCHNP50CA097190 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Greg M. Delgoffe · 2004 to 2026
$49.6M
STAT-Mediated TGFalpha EGFR Signaling in squamous cell carcinoma of head neckR01CA077308 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI GRANDIS, JENNIFER RUBIN · 1998 to 2018
$5.0M
NCI NIH HHS P50 CA097190NCI NIH HHS R01 CA077308
6 · The paper itself

Abstract

backgroundHyperactivation of STAT3 via constitutive phosphorylation of tyrosine 705 (Y705) is common in most human cancers, including head and neck squamous carcinoma (HNSCC). STAT3 is rarely mutated in cancer and the (epi)genetic alterations that lead to STAT3 activation are incompletely understood. Here we used an unbiased approach to identify genomic and epigenomic changes associated with pSTAT3(Y705) expression using data generated by The Cancer Genome Atlas (TCGA). METHODS AND

findingsMutation, mRNA expression, promoter methylation, and copy number alteration data were extracted from TCGA and examined in the context of pSTAT3(Y705) protein expression. mRNA expression levels of 1279 genes were found to be associated with pSTAT3(705) expression. Association of pSTAT3(Y705) expression with caspase-8 mRNA expression was validated by immunoblot analysis in HNSCC cells. Mutation, promoter hypermethylation, and copy number alteration of any gene were not significantly associated with increased pSTAT3(Y705) protein expression.

conclusionsThese cumulative results suggest that unbiased approaches may be useful in identifying the molecular underpinnings of oncogenic signaling, including STAT3 activation, in HNSCC. Larger datasets will likely be necessary to elucidate signaling consequences of infrequent alterations.

Indexed as

GenomicsCarcinoma, Squamous CellCaspase 8Cell Line, TumorDNA Copy Number VariationsDNA MethylationFemaleGene Expression Regulation, NeoplasticHead and Neck NeoplasmsHumansMaleMiddle AgedMutation RatePhosphorylationPromoter Regions, GeneticProtein Array AnalysisCaspase 8RNA, MessengerSTAT3 Transcription Factor

Identifiers

PMID27855189
PMCPMC5113908
OpenAlexW2552587176

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.