ArticleScientific reports2016
The archaeal "7 kDa DNA-binding" proteins: extended characterization of an old gifted family.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
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- Influence of Subcellular Localization on the Cytotoxicity of Targeted α-Therapy.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2026Article
- Development of potent Affitin-based bispecific NK cell engagers for the therapy of MSLN-expressing cancers.Molecular therapy. Oncology · 2025Article
- Engineered chimeric T7 RNA polymerase improves salt tolerance and reduces dsRNA impurity generation during in vitro transcription of mRNA.Nucleic acids research · 2025Article
- Predicting sequence-specific amplification efficiency in multi-template PCR with deep learning.Nature communications · 2025Article
- Review
- DNA binding and mitotic phosphorylation protect polyglutamine proteins from assembly formation.Cell · 2025Article
- The molecular basis for DNA-binding by competence T4P is distinct in a representative Gram-positive and Gram-negative species.PLoS pathogens · 2025Article
- Efforts to Downsize Base Editors for Clinical Applications.International journal of molecular sciences · 2025Review
- The Lrs14 family of DNA-binding proteins as nucleoid-associated proteins in the Crenarchaeal order Sulfolobales.Molecular microbiology · 2025Review
- Effects of genomic location on ectopic integration and gene expression of a reporter gene cassette inFrontiers in microbiology · 2025Article
- Engineering miniature IscB nickase for robust base editing with broad targeting range.Nature chemical biology · 2024Article
- Mycobacteriophage Alexphander GeneInternational journal of molecular sciences · 2024Article
- Engineering miniature CRISPR-Cas Un1Cas12f1 for efficient base editing.Molecular therapy. Nucleic acids · 2024Article
- Robust miniature Cas-based transcriptional modulation by engineering Un1Cas12f1 and tethering Sso7d.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Small Proteins in Archaea, a Mainly Unexplored World.Journal of bacteriology · 2022Review
- Evaluation of docking procedures reliability in affitins-partners interactions.Frontiers in chemistry · 2022Article
- Application of Affitins for Affinity Purification of Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The "7 kDa DNA-binding" family, also known as the Sul7d family, is composed of chromatin proteins from the Sulfolobales archaeal order. Among them, Sac7d and Sso7d have been the focus of several studies with some characterization of their properties. Here, we studied eleven other proteins alongside Sac7d and Sso7d under the same conditions. The dissociation constants of the purified proteins for binding to double-stranded DNA (dsDNA) were determined in phosphate-buffered saline at 25 °C and were in the range from 11 μM to 22 μM with a preference for G/C rich sequences. In accordance with the extremophilic origin of their hosts, the proteins were found highly stable from pH 0 to pH 12 and at temperatures from 85.5 °C to 100 °C. Thus, these results validate eight putative "7 kDa DNA-binding" family proteins and show that they behave similarly regarding both their function and their stability among various genera and species. As Sac7d and Sso7d have found numerous uses as molecular biology reagents and artificial affinity proteins, this study also sheds light on even more attractive proteins that will facilitate engineering of novel highly robust reagents.
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