ArticleScientific reports2016
Labeling and analysis of chicken taste buds using molecular markers in oral epithelial sheets.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Taste Preferences in Broilers: Effect of Age, Delivery Matrix, and Number of Chickens per Pen on Selection and Consumption Behaviour.Animals : an open access journal from MDPI · 2024Article
- The tongue of the red-eared slider (Trachemys scripta elegans): morphological characterization through gross, light, scanning electron, and immunofluorescence microscopic examination.BMC veterinary research · 2024Article
- The avian taste system.Frontiers in physiology · 2023Review
- Oral expressions and functional analyses of the extracellular calcium-sensing receptor (CaSR) in chicken.Scientific reports · 2022Article
- Bitter Taste Perception in Chickens.The journal of poultry science · 2022Article
- The umami receptor T1R1-T1R3 heterodimer is rarely formed in chickens.Scientific reports · 2021Article
- Abundant proliferating cells within early chicken taste buds indicate a potentially "built-in" progenitor system for taste bud growth during maturation in hatchlings.Histology and histopathology · 2019Article
- Bitter taste receptor T2R7 and umami taste receptor subunit T1R1 are expressed highly in Vimentin-negative taste bud cells in chickens.Biochemical and biophysical research communications · 2019Article
- Bitter Taste Sensitivity and the Expression of Bitter Taste Receptors at Different Growth Stages of Chicks.The journal of poultry science · 2018Article
- An Update on the Sense of Taste in Chickens: A Better Developed System than Previously Appreciated.Journal of nutrition & food sciences · 2018Article
- RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis.Scientific reports · 2017Article
- From Cell to Beak: In-Vitro and In-Vivo Characterization of Chicken Bitter Taste Thresholds.Molecules (Basel, Switzerland) · 2017Article
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Authors and funding
12 authors.
Funding
Abstract
In chickens, the sensory organs for taste are the taste buds in the oral cavity, of which there are ~240-360 in total number as estimated by scanning electron microscopy (SEM). There is not an easy way to visualize all taste buds in chickens. Here, we report a highly efficient method for labeling chicken taste buds in oral epithelial sheets using the molecular markers Vimentin and α-Gustducin. Immediate tissue fixation following incubation with sub-epithelially injected proteases enabled us to peel off whole epithelial sheets, leaving the shape and integrity of the tissue intact. In the peeled epithelial sheets, taste buds labeled with antibodies against Vimentin and α-Gustducin were easily identified and counted under a light microscope and many more taste buds, patterned in rosette-like clusters, were found than previously reported with SEM. Broiler-type, female-line males have more taste buds than other groups and continue to increase the number of taste buds over stages after hatch. In addition to ovoid-shaped taste buds, big tube-shaped taste buds were observed in the chicken using 2-photon microscopy. Our protocol for labeling taste buds with molecular markers will factilitate future mechanistic studies on the development of chicken taste buds in association with their feeding behaviors.
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